Borderline high serum free light chain kappa/lambda ratios are seen not only in dialysis patients but also in non-dialysis-dependent renal impairment and inflam- matory states. Am J Clin Pathol 2009;132:309.Marshall G, Tate J, Mollee P. Borderline high serum free light chain kappa/lambda ...
(w/v) dried milk powder in PBS, prior to adding scAbs or mAbs in doubling dilution. The binding was detected using HRP-conjugated anti-human kappa light chain antibody (for scAbs; Sigma, #A7164) and HRP-conjugated anti-mouse IgG (for mAbs; Sigma, #A6782) as the secondary antibody and...
A series of detection scAbs recognising various tau epitopes were added at 10 µg/mL and detected using HRP-conjugated anti-human kappa light chain antibody as described above. LLOQ was calculated as the lowest dilution in the standard curve with signal higher than the average blank value plus...
While its mother brand Robe di Kappa was founded in 1916, Kappa, as we know it today, began life in 1967. When we say "Kappa as we know it", we mean Kappa in its legend of sportswear propensity. With one of the most memorable logos ever — need "Omini" — Kappa made a splash ...
The full-length humanized 10G4 monoclonal antibody was produced from transient 293-F cells (Invitrogen) using a 3:1 light:heavy chain ratio of plasmid. Cultures were collected after 5 days. Antibodies were purified by protein-A affinity chromatography using MabSelect resin, dialyzed against 1× ...
Normal saline: 0.9% (w/v) NaCl. The ratio of IGg1/IGg2a reflects the dominant response type in the body, and IgG2 can interact with FcRI, FcRII, FcRIII, and FcRIIV (Beers et al., 2016). Thus, IgG2a antibodies should be more effective than IgG1 antibodies in clearing viral ...
Hence, the anti-inflflammatory activity of lupeol could be explained by the suppression of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) expression in keratinocytes and the regulation of MMP-2 (matrix metalloproteinase-2) [4...
In general, that response involves the following steps: first, toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) to initiate the antiviral innate immune response [152,153]; next, the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and ...
IgG isotype, and the EQ-λV-LEADER-17mix was used to fill in missing lambda sequences. EQ-κ primers were used to search for possible kappa light chains, while EQ-HV screening primers were used in the initial setup to look for non-dominant HV-gene representatives in bulk sorted cells....
(1) Background: Upfront treatment consolidation with high-dose chemotherapy (HDCT) and autologous stem cell transplantation (ASCT) has relevantly contributed to achieving durable remissions following induction treatment in multiple myeloma (MM) patients.