Learn more about pushing the limits of Golden Gate Assembly (50+ fragment assembly achievable with high efficiency and accuracy!)
Golden Gate Assembly Protocol for Using NEB Golden Gate Assembly Mix (E1600) Transformation Protocol for Using NEB Golden Gate Assembly Mix (E1600) Recommended Screening Protocols for Using NEB Golden Gate Assembly Mix (E1600) Golden Gate (24 Fragment) Assembly Protocol Golden Gate Assembly Protocol...
无缝克隆有两个主要的流派:Gibson Assembly和Golden Gate Assembly。目前市面上比较具有代表性的无缝克隆试剂有:NEB的Gibson Assembly、NEBuilder Assembly和Golden Gate Assembly;宝生物的In-Fusion和In-Fusion Snap Assembly 。除Golden Gate Assembly外,其余无缝克隆试剂的基本原理实际上都和早期的Gibson Assembly的是一样...
BsmBI-v2 has been engineered by NEB and outperforms BsmBI in Golden Gate Assemblies. Together these enzymes can direct the assembly of multiple inserts/modules and also single insert/library generation cloning with single insert(s) using the Golden Gate approach. The pGGAselect destination plasmid...
在连接反应中,粘性末端的碱基数越多,连接产物的保真度越高,因此Golden Gate Assembly通常采用能够产生4碱基粘性末端的IIS限制酶,常用的有BsaI、BsmBI和BbsI (图2)。NEB公司对这三种野生型的限制酶进行了分子改造,得到三种识别序列不变但特异性更强的限制酶,分别是BsaI-HFv2、BsmBI-v2和BbsI-HF (图2)。这些限制...
NEB Golden Gate组装试剂盒(BsmBI-v2)包含: –NEB® Golden Gate酶预混液 –T4 DNA连接酶反应缓冲液(10X) –pGGAselectII DNA 用于Golden Gate的IIS型限制性内切酶 –BsaI (NEB #R0535) –BsaI-HFv2 (NEB #R3733) –BbsI (NEB #R0539)
NEB 10-beta E. coli 感受态细胞(高效级) Q5热启动超保真2 X预混液 特性: 使用新酶BsaI-HFv2(针对Golden Gate应用优化) 无缝克隆–组装后无额外碱基存在 目的质粒含T7/SP6启动子 单次反应即可按顺序组装多个片段(2-20+) 也可用于单个片段克隆和文库制备 ...
NEB Golden Gate组装试剂盒(BsmBI-v2)包含: –NEB® Golden Gate酶预混液 –T4 DNA连接酶反应缓冲液(10X) –pGGAselectII DNA 用于Golden Gate的IIS型限制性内切酶 –BsaI (NEB #R0535) –BsaI-HFv2 (NEB #R3733) –BbsI (NEB #R0539)
NEB Golden Gate组装试剂盒(BsmBI-v2)包含: –NEB®Golden Gate酶预混液 –T4 DNA连接酶反应缓冲液(10X) –pGGAselectII DNA 用于Golden Gate的IIS型限制性内切酶 –BsaI (NEB #R0535) –BsaI-HFv2 (NEB #R3733) –BbsI (NEB #R0539) –BbsI-HF (NEB #R3539) ...
NEB 10-beta E. coli 感受态细胞(高效级) Q5热启动超保真2 X预混液 特性: 使用新酶BsaI-HFv2(针对Golden Gate应用优化) 无缝克隆–组装后无额外碱基存在 目的质粒含T7/SP6启动子 单次反应即可按顺序组装多个片段(2-20+) 也可用于单个片段克隆和文库制备 ...