Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
Golden Gate Assembly Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Transformation Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Recommended Screening Protocols for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) 应用实...
NEBridge® Golden Gate Assembly , Restriction Enzyme Digestion, Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 R3733V-20 ...
Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
NEB® Golden Gate Assembly Kit (BsaI-HF®v2)E1601L100 rxns¥4,799¥3,599 E1601S20 rxns¥1,799¥1,349 T7 Endonuclease IM0302L1,250 units¥3,149¥2,204 M0302S250 units¥789¥552 EnGen Mutation Detection KitE3321S25 rxns¥2,399¥1,799 ...
Golden Gate Assembly of 35 fragments using BsmBI- v2 限制酶与 T4 DNA连接酶,随后转化为 NEB 10-beta CompetentE。大肠杆菌(高效)。 材料 NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602)pGGAselect Destination Plasmid*NEB 10-beta CompetentE.大肠杆菌(NEB #C3019)NEB 10-beta/稳定...
evolution (7).Please note that while general descriptions regarding Golden Gate Assembly use the BsmBI nomenclature, this kit and protocols feature the specific engineered form optimized for Golden Gate Assembly, BsmBI-v2.To learn more about the Golden Gate Assembly workflow, watch this video ...
NEBuilder HiFi DNA Assembly Master Mix E2621X 250 rxns ¥26,259 ¥19,694 E2621L 50 rxns ¥5,989 ¥4,492 E2621S 10 rxns ¥1,519 ¥1,139 NEB® Golden Gate Assembly Kit (BsaI-HF®v2) E1601L 100 rxns ¥4,799 ¥3,599 E1601S 20 rxns ¥1,799 ¥1,349...
BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules) Fast cloning experiments with 5-minute ligation step Simplified screening with low/no colony background and no blue/white selection required ...
Closer to the clinic: Optimized DNA assembly for personalized medicine research Featured Webinar Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protoco...