Learn more about pushing the limits of Golden Gate Assembly (50+ fragment assembly achievable with high efficiency and accuracy!)
Transformation Protocol for Using NEB Golden Gate Assembly Mix (E1600) Recommended Screening Protocols for Using NEB Golden Gate Assembly Mix (E1600) Golden Gate (24 Fragment) Assembly Protocol Golden Gate Assembly Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Tr...
NEBridge® Golden Gate Assembly , Restriction Enzyme Digestion, Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 R3733V -20 BsaI-HF®v2 R3733VVIAL -20 1 x 0.025 ...
NEBridge® Golden Gate Assembly 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 M1100S -20 NEBridge® Ligase Master Mix M1100SVIAL -20 1 x 0.25 ml 3 X M1100L -20 NEBridge® Ligase Master Mix M1100LVIAL -20 1 x 1.25 ml 3 X 特性和用法 需要但...
Restriction Enzymes in Golden Gate Assembly Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of TypeIIS enzymes. Restriction Enzymes in Isothermal Amplification Isothermal am...
NEBridge Golden Gate Assembly Tool can be used to design primers for Golden Gate DNA Assembly reactions, estimate overhang fidelity, or find optimal Golden Gate break points for long sequences.
Golden Gate Assembly of 35 fragments using BsmBI- v2 限制酶与 T4 DNA连接酶,随后转化为 NEB 10-beta CompetentE。大肠杆菌(高效)。 材料 NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602)pGGAselect Destination Plasmid*NEB 10-beta CompetentE.大肠杆菌(NEB #C3019)NEB 10-beta/稳定...
NEBridge® Golden Gate Assembly 产品组分信息 本产品提供以下试剂或组分: 特性和用法 需要但不提供的材料 用户定义的 DNA 片段 用户选择的 NEB IIS 型限制性内切酶 感受态细胞 其它转化材料 优势和特性 Features 经优化设计,适用于方便、高效、准确的 Golden Gate 组装 ...
Golden Gate组装通常用于一步组装5 - 10个DNA片段,其局限性在于DNA连接酶可能会连接一些不匹配的序列,导致错误,重复或减少组装片段。 NEB公司Gregory J. S. Lohman等人发表在ACS Synthetic Biology上的一篇“Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design”报道,他们通过使用...
This tool is ideal for NEB Q5® and NEBuilder® HiFi DNA Assembly applications. Application Note Learn about an accessible method for shuffling DNA using NEBridge® Golden Gate Assembly to recombine proteins and find new properties. Application Note Read about how NEB scientists extract and...