BsaI-HFv2 的特异性与 BsaI(NEB #R0535)相同,但经过改造后星号活性降低。 某些dcm 甲基化与酶切位点的重叠组合影响酶切,某些 CpG 甲基化与酶切位点的重叠组合阻断酶切。 操作说明、说明书 & 用法 操作说明 优化限制性内切酶反应 Golden Gate (24 Fragment) Assembly Protocol ...
Golden Gate (24 Fragment) Assembly Protocol Golden Gate Assembly Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Transformation Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Recommended Screening Protocols for Using NEBridge®Golden...
NEB Golden Gate组装试剂盒(BsaI-HFv2)内含优化的混合液。利用Golden Gate方法,混合液中的BsaI-HFv2和T4 DNA连接酶可以完成多个片段的组装。试剂盒自带目的质粒pGGA,为组装提供骨架,同时为亚克隆提供限制性内切酶酶切位点,以及为体外转录提供T7/SP6启动子序列。 Golden Gate可高效、无痕地克隆组装DNA片段。该方法起...
NEB Golden Gate组装试剂盒(BsaI-HFv2)内含优化的混合液。利用Golden Gate方法,混合液中的BsaI-HFv2和T4 DNA连接酶可以完成多个片段的组装。试剂盒自带目的质粒pGGA,为组装提供骨架,同时为亚克隆提供限制性内切酶酶切位点,以及为体外转录提供T7/SP6启动子序列。 Golden Gate可高效、无痕地克隆组装DNA片段。该方法起...
NEB Golden Gate组装试剂盒(BsaI-HFv2)内含优化的混合液。利用Golden Gate方法,混合液中的BsaI-HFv2和T4 DNA连接酶可以完成多个片段的组装。试剂盒自带目的质粒pGGA,为组装提供骨架,同时为亚克隆提供限制性内切酶酶切位点,以及为体外转录提供T7/SP6启动子序列。
Overview Golden Gate Assembly of 24 fragments using BsaI-HFv2 restriction enzyme with T4 DNA Ligase and subsequent transformation into NEB 10-beta Competent E
Which restriction enzymes are used in Golden Gate Assembly? Which restriction enzymes are used in GoldenBraid Assembly? Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)? What is the activity of the Type IIS restriction enzyme BsaI-HFv2 (NEB #R3733) in T4 DNA Liga...
EnGen sgRNA Synthesis Kit, S. pyogenes E3322S 20 rxns ¥4,799 ¥3,599 NEBuilder HiFi DNA Assembly Master Mix E2621X 250 rxns ¥26,259 ¥19,694 E2621L 50 rxns ¥5,989 ¥4,492 E2621S 10 rxns ¥1,519 ¥1,139 NEB® Golden Gate Assembly Kit (BsaI-HF®v2) ...
NEBuilder HiFi DNA Assembly Master Mix E2621X 250 rxns ¥26,259 ¥19,694 E2621L 50 rxns ¥5,989 ¥4,492 E2621S 10 rxns ¥1,519 ¥1,139 NEB® Golden Gate Assembly Kit (BsaI-HF®v2) E1601L 100 rxns ¥4,799 ¥3,599 E1601S 20 rxns ¥1,799 ¥1,349...
28 PvuI-HF(星选酶) 支 2 R3150L 29 Golden Gate Assembly Kit (BsmBI-v2) 支 2 E1602L 30 Golden Gate BsaI-HF? v2 支 3 E1601S 31 Q5? High-Fidelity 2X Master Mix 支 6 M0492L 32 Exonuclease VIII 支 2 M0545S 33 ATP 支 2 P0756S 34 MmeI 支 4 R0637S 35 T7E1 支 4 E3321 ...