Complement this kit with NEBridge Golden Gate Assembly Kit (BsaI-HFv2) (NEB #E1601) Product Introduction Learn about ligase fidelity and push the limits of your Golden Gate Assembly Reactions (50+ fragments) Uses BsmBI-v2, which is optimized for Golden Gate Assembly Use for seamless cloning ...
The NEBridge Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. Also included is the pGGAselect destination plasmid, which provides a backbone...
Golden Gate Assembly Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Transformation Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) Recommended Screening Protocols for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) 应用实...
1.最常规的ligation 2.之前的CRISRP KO gRNA用的是Golden Gate Assembly NEBridge® Golden Gate Assembly Kit (BsmBI-v2) Golden Gate Assembly Protocol for using NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) 3.现在RNAi所用的shRNA则需要用Gibson Assembly Gibson Assembly® Gibson...
Nebridge Golden Gate Assembly Kit Bsmbi V2 Reaction Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
The NEBridge Golden Gate Assembly Kit (BsmBI-v2) contains an optimized mix of BsmBI-v2 and T4 DNA Ligase. BsmBI-v2 has been engineered by NEB and outperforms BsmBI in Golden Gate Assemblies. Together these enzymes can direct the assembly of multiple inserts/modules and also single insert/...
Learn how to push the limits of your Golden Gate Assembly, and try one of our convenient kits (using BsmBI-v2 or BsaI-HFv2) The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate Assembly (1,2), has its origins in 1996, when for the first time it...
图1植物细胞器基础编辑器Golden Gate assembly system。 为此,首先开发了Golden Gate assembly system来构建叶绿体靶向DdCBE(cp-DdCBE)质粒或线粒体靶向DdCBE(mt-DdCBE)质粒(图.1)。表达质粒编码由叶绿体转运肽(CTP)或线粒体靶向序列(MTS)、TALE N端或C端结构域、分裂的(DddAtox半体(G1333N、G1333C、G1397...
out and assembled in tandem into the ‘‘array’’plasmids by BsaI-based Golden Gate Cloning(Fig. 1, step 2).The array backbone plasmids (spectinomycin resistance)used for the second step assembly are pFUS-B 1 to pFUS-B 10,pFUS-A,pFUS-A30A,and pFUS-A30B of the TALEN assembly kit...
golden_gate_talen_assembly_v7[1] Golden Gate TALEN assembly This is an expanded and slightly modified TAL assembly protocol published in the original form in Cermak, et al., 2011 (http://dx.doi.org/10.1093/nar/gkr218) Modifications to the published protocol by Michelle Christian, Colby ...