SensitivityThe minimum detectable dose of this kit is typically less than 34pg/mL. Specificity This assay has high sensitivity and excellent specificity for detection of Deoxyribonuclease I (DNASE1). No significant cross-reactivity or interference between Deoxyribonuclease I (DNASE1) and analogues was ...
After DNAse I treatment, CHO and YAC-IRS had an intensity of 85% but IRS-20 had an intensity of 60%, when compared with the controls. DNAse I sensitivity differences between the cell lines indicate that overall conformation of chromatin might contribute to radiation sensitivity of the IRS-20...
At the same time, the sensitivity of DNA from different rat liver to DNase Ⅰ is mersured. The results demonstrate that DNA methylase activity of rat liver of Diabetic Nephropathy is obviously lower than the control, and its genome DNA possesses higher sensitivity to Dnase I than that of ...
DNase I footprinting was first published in 1978 and predates both Sanger sequencing and NGS. The first published use with NGS was published by Boyle et al. and later optimized for sequencing1. A high-sensitivity protocol is also available (scDNase-seq)2. ...
Using DNase I digestions of intact nuclei in conjunction with NGS, known as DNase- seq, allowed for genome-wide identification of DHSs with unmatched specificity, sensitivity, and throughput [6]. The improved quality of NGS data has made DNase- seq a preferred method of cho...
sensitivity for identifying driver genes8,9,10. In this study, we have analyzed whole genome sequences for 657 breast cancer samples and more than one thousand tumors across 19 additional cancer types to detect non-coding driver mutations. We focused on analyzing DNase I hypersensitive sites (...
These results indicate that the fluorescence-based assay can determine the sensitivity of EBV DNase to high ionic strengths. Taken together, these results suggest that the fluorescence-based nuclease activity assay can be used to detect the preferred environmental conditions for EBV DNase activity. ...
1a, “Protocol: Ramani et al. + biotin fill-in”). Thus, we recommend using no adapters and employing a biotin fill-in strategy to prepare DNase Hi-C libraries. Quality control 1. If adapters were used for end-labelling, then a ligation assay was performed to ensure that the ...
AptasensorPatulin (PAT) is a mycotoxin that exists in a wide range and is harmful to human health, which has aroused great attention to food safety. In this study, we established a novel assay to detect food samples contaminated by PAT. First of all, we proposed a truncation protocol based...