DNase I hypersensitivity assayNext generation DNA sequencingDNase-seqChromatin structureDNase hypersensitivity (DHS) analysis coupled with high-throughput DNA sequencing (DNase-seq) has emerged as a powerful tool to analyze chromatin accessibility and identify regulatory sequences in genomic DNA on a ...
Tx gene is the DNA sequence with transforming activity which was cloned from the epithelial cell line(CNE2) of human nasopharyngeal carcinoma(NPC). The goal of this study is to find transcriptional activated site on the Tx 2.8kb fragment using DNase-Ⅰ hypersensitivity assay, by which understandi...
containing either the wild-type or mutated DHS attached to a minimal promoter36and GFP. These constructions were electroporated intoC.intestinalisfertilized eggs to assay their function. The two GATA mutations result in significant differential expression, with one resulting in overexpression and one in...
1. Decellularized porcine heart valves were prepared by using Trypsin-ethylenediamine tetraacetic acid andDNase. 利用胰酶-乙二胺四乙酸、核酸酶处理猪心瓣膜制备脱细胞支架。 2. TheDNasehypersensitivity assay is higher in resolution at the DNA level and can be done on a large number of cell types sin...
Genome-scale mapping of DNase I hypersensitivity. Curr Protoc Mol Biol. 2013;Chapter 27:Unit 21 7. 8. Zhang W, Wu Y, Schnable JC, Zeng Z, Freeling M, Crawford GE, et al. High- resolution mapping of open chromatin in the rice genome. Genome Res. 2012;22(1...
In order to describe the change in chromatin hypersensitivity, DHS change scores (∆DHS) were calculated between the two conditions using a slightly modified formula to the one introduced by He et al. [61]. The DHS change score for i:th DHS was calculated using the following formula: $$...
In order to describe the change in chromatin hypersensitivity, DHS change scores (∆DHS) were calculated between the two conditions using a slightly modified formula to the one introduced by He et al. [61]. The DHS change score for i:th DHS was calculated using the following formula: ...
We also examined the chromatin structure of the PPE gene in different tissues using the DNase hypersensitivity assay. Two major DNase-hypersensitive sites were detected at -1800 and -200 bases upstream of exon 1. The -200 site was present in nuclei isolated from tissues which express the PPE ...
The chromatin structure of the ELP gene was analyzed by DNase I hypersensitivity. A strong DNase I hypersensitive site (Y1DH) was identified at the exon 1 region of the ELP gene in steroidogenic Y1 cells. A strong DNase 1 hypersensitive site (ECDH2) downstream of the exon 8 of the ELP ...
DNase I hypersensitivity (DHS) combined with next generation sequencing ( DNase -seq) is an efficient way of observing, in a single experiment, the genome-wide chromatin effects associated with the binding of multiple transcription factors. Using quantitative contrasts of DHS before and after ...