Protocol for DNAse I treatment: Combine 1 µg total RNA, 1 µL 10X DNAse I buffer (200 mM Tris-HCl (pH 8.4), 500 mM KCl, 20 mM MgCl2), 1 µL Amplification Grade DNAse I (1 unit/µL), and DEPC-treated water to 10 µL. Incubate for 15 min at room temperature. Inacti...
For another 10 samples, [beta]-globin DNA was not detected in any samples after we used on-column DNase digestion with 80 [micro]L of enzymebuffer mixture from the RNase-Free DNase Set (Qiagen) for the columnbased protocol and postextraction DNase treatment with a DNase I reagent set (Inv...
RNAse-free DNase treatment of the RNA can reduce DNA to undetectable levels. We recommend using our DNase 1, Amplification Grade (Cat. No. 18068015). Add of 1 µL of 25 mM EDTA solution to the reaction mixture in 10 µL reaction with 1 unit DNase I, Amplification Grade (or 1:1 ...
DNase I treatment Nuclei were incubated at 37°C for 3 min with limiting concentrations of DNase I enzyme in buffer A supplemented with Ca2+. The reaction was terminated with an equal volume of stop buffer (50 mM Tris-HCl, 100 mM NaCl, 0.1% SDS, 100 mM EDTA, 1 mM spermidine, 0.5 sp...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free Includes Enzyme Incubation Buffer Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits; Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free; Includes Enzyme Incubation Buffer; Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1...
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify. Q6: Is DNase I treatment necessary? If the downstream application requires DNA-free RNA, we would recommend performing in...
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify. Q6: Is DNase I treatment necessary? If the downstream application requires DNA-free RNA, we would recommend performing in...
In addition, DNase-seq has been highly effective in comparing transcription factor (TF) binding profiles in treatment conditions relative to control conditions.Housheng Hansen HeClifford A MeyerSheng'en Shawn HuMei-Wei ChenChongzhi ZangYin LiuPrakash K RaoTeng FeiHan XuHenry LongX Shirley LiuMyles ...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free Includes Enzyme Incubation Buffer Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600...