faecalis suspensions were mixed with equal volumes of DNase I (Sigma Aldrich, Br[??]ndby, Denmark; final concentration of 50 Kunitz/mL, 5 mM [MgCl.sub.2] in 0.9% NaCl) or heat-inactivated DNase I (100[degrees]C, 30 min), injected in 96-well plates (Ibidi [mu]-plate uncoated, Pl...
Notably, our results showed that high-quality Hi-C libraries could not be obtained in this case (see the metrics of Protocol: Ma et al. in Fig. 1). Fig. 2 Chromatin digestion and ligation in DNAse Hi-C experiments. a) Reproducibility of chromatin digestion by DNase I under the ...
The members of the human β globin gene family are flanked by strong DNase I hypersensitive sites. The collection of sites 5′ to the ϵ globin gene is able to confer high levels of expression of linked globin genes, but a function has not been assigned to the site 3′ to the β glo...
Sigma-Aldrich R6148 RNase A solution View Price and Availability Roche 04536282001 DNase I recombinant View Price and Availability Properties biological source bovine pancreas Quality Level 100 form solution specific activity ≥30 units/mg protein packaging pkg of 500 μg (1 ml) manufacturer/tradename...
For immunoelectron microscopy assay, mature trophozoite-infected erythrocytes (P. falciparum3D7 andP. bergheiANKA) were fixed with 0.2% v/v glutaraldehyde and 1% w/v paraformaldehyde in PBS for 30 min at 4 °C. The samples were embedded in LR white resin (Sigma) at 50 °C for ...
c—control samples incubated without enzymes. One unit of enzyme activity of DNase I according to the supplier instruction completely degrades 1 μg of plasmid DNA in 10 min at 37 °C (EN0525, Thermo Waltham MA, USA); 1 U of α-amylase from Bacillus sp. (A6380, Sigma, St. Louis, ...
(Sigma-Aldrich, St. Luis, MO, USA) were used for most of the genes, and the LightCycler FastStart DNA Master SYBR Green I assay (Roche) for the rest. Roche’s Reference Gene Assay for HPRT was used for normalization. Primer sequences and probe information are given in Additional file9:...