DNase I(扩增级)将单链和双链 DNA 酶切成含 5' 磷酸的寡脱氧核糖核苷酸。DNase I(扩增级)适用于在关键 RNA 净化程序(如 RNA-PCR 扩增前的程序)中消除 DNA。经纯化和检测,RNase 污染水平无法检出。通过使用 RNA 分子量标准品进行核糖核酸酶检测确定无 RNase。
3095635Certificate of Analysis2024年10月30日AM2239 显示5 条结果, 在上面搜索特定证书 申请COA 安全数据表 SDS Product Information PI Sheet: TURBO DNase 常见问题解答 (FAQ) When using the MagMAX-96 for Microarrays Total RNA Isolation Kit modified spin protocol (https://www.thermofisher.com/us/en/...
2.5 TURBO DNA-free treated/RT-PCR 2 Untreated/RT-PCR 1.5 Untreated/RT-minus 1 .5 0 TURBO DNA-free treated/RT-minus -.5 10 20 30 40 Cycle Number Table 1 Treatment of RNA with TURBO DNA-free™ maintains target sensitivity in real-time RT-PCR. Total RNA from HeLa S3 cells was...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free Includes Enzyme Incubation Buffer Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600...
If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.Q6: Is DNase I treatment necessary? If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment....
For another 10 samples, [beta]-globin DNA was not detected in any samples after we used on-column DNase digestion with 80 [micro]L of enzymebuffer mixture from the RNase-Free DNase Set (Qiagen) for the columnbased protocol and postextraction DNase treatment with a DNase I reagent set (Inv...
Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes with electrophoresis. DNase treatment makes the mixture more soluble, even if ...
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify. Q6: Is DNase I treatment necessary? If the downstream application requires DNA-free RNA, we would recommend performing in...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free Includes Enzyme Incubation Buffer Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600...
Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits; Also includes protocol for digestion in-solution followed by RNA Clean-Up Guaranteed RNase-Free; Includes Enzyme Incubation Buffer; Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1...