DNase I protection assay of non-culturable Y. pestis cells from the autoclaved tap water microcosm compared to actively growing Y. pestis.doi:10.1371/JOURNAL.PONE.0017585.T001R. DavidJ. DanielR. AmyHowlett AmySiebert GretchenJ. K. Richard...
用途:用于RNA合成,合成的RNA可以用于或用作:杂交探针,基因组DNA序列分析,核糖核酸酶保护测定(RNase protection assay),反义RNA合成,作为体外翻译的RNA模板,RNA剪接研究的底物,RNA二级结构和RNA-蛋白质相互作用,核酸扩增分析,siRNA、miRNA等小RNA。 活性定义: 37℃60分钟内,催化1 nmol AMP掺入到多聚核苷酸中所需的...
the binding site is protected from enzymatic cleavage. This protection will result in a clear area on the gel which is referred to as a “footprint”. By varying the concentration of the DNA-binding protein, the binding affinity of the protein can be estimated according to the minimum concent...
A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding ... S Kim,SH Kim,J Ahn,... - 《Moleculer Cells》 被引量: 0发表: 2019年 In vivo genomic footprinting of thyroid hormone-responsive gene...
A – Pattern of DNase I protection: lane G –MaSat G cleavage Maxam-Gilbert reaction; lane 1 – free MaSat fragment cleaved by DNase; lane 2 - MaSat-SAF-A complex cleaved by DNase. Protected residues are marked by arrowheads at the top. The sequence is indicated at the bottom. The ...
protection as a gap in the otherwise continuous background of digestion products (for examples,seeFig. 1). The technique can be used to determine the site of interaction of most sequence-specific DNAbinding proteins but has been most extensively applied to the study of transcription factors. ...
We further investigated the significance of TatD-specific anti- bodies in host protection against parasite infection in vivo. BALB/ c mice (N ¼ 15 in each group) were immunized four times with the His-tagged recombinant P. berghei TatD-like DNase protein (rPbTatD-HIS) (Fig. 6a) and...
We used LuxR concentrations varying over 4 orders of magnitude in the DNase I protection assay to measure the LuxR-lux box DNA dissociation constant (Fig. 3B). Under our experimental conditions, 0.1...Brenowitz M, Senear DF, Kingston RE (2001) DNase I footprint analysis of protein–DNA ...
Deoxyribonuclease I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I-catalyzed hydrolysis. Binding sites are ...
These differences of the DNase I hypersensitive sites may be related to the differential expression of ELP isoforms in Y1 cells and EC cells. In addition, the gel shift assay and DMS protection assay revealed that ELP binds to the ECDH2 region....