The resultant combination is incubated under conditions whereby the receptor is bound to the ligand and, but for the presence of said candidate pharmacological agent, the transcription factor is sequence-specifically bound to the nucleic acid. Unbound transcription factor is then removed or washed from...
Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1 Science, 238 (1987), pp. 1386-1392 CrossrefView in ScopusGoogle Scholar Brayer and Segal, 2008 K.J. Brayer, D.J. Segal Keep your fingers off my DNA: protein-pro...
Such DNA sequences are recognized by transcription factors (TFs) that recruit the transcriptional machinery. Achievement of specific sequence recognition is not a trivial problem; many TFs recognize similar consensus DNA-binding sites and a genome can harbor thousands of consensus or near-consensus ...
Usually, the DNA-binding domain forms a module that can be separated from the rest of the transcription factor structure without losing activity. This facilitates structural studies of the isolated DNA-binding domains or their complexes with DNA using techniques that require low molecular weight ...
We have cloned and characterized the rat gene that encodes the p48 DNA-binding subunit of pancreas transcription factor 1 (Ptf1), a cell-specific basic reg... Krapp,A. - 《Journal of Biological Chemistry》 被引量: 20发表: 1996年 The bHLH protein PTF1-p48 is essential for the formation...
Volume: 8, P: 225 Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathways In this work, high-throughput microfluidic assays reveal how allosteric substitutions that alter folding-and-binding pathways shape DNA specificity and selectivity landscap...
TF transcription factor, PWM position weight matrix. Also see Supplementary Figs. 2, 3, and Supplementary Data 4. Full size image The dimeric binding type could be further separated into three subtypes given different monomer orientations, including head-to-head, head-to-tail, and tail-to-tail...
The HMM library obtained can be used to classify any newly discovered transcription factor according to its DNA-binding domain and, thus, to generate hypotheses about its DNA-binding specificity. (Copyright 2004 Japanese Society for Bioinformatics) 展开 ...
Okamura H, Aramburu J, Garcia-Rodriguez C, Viola JP, Raghavan A, Tahiliani M, Zhang X, Qin J, Hogan PG, Rao A: Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity. Mol Cell. 2000, 6: 539-550. Article CAS Pub...
The HMM library obtained can be used to classify any newly discovered transcription factor according to its DNA-binding domain and, thus, to generate hypotheses about its DNA-binding specificity. (Copyright 2004 Japanese Society for Bioinformatics)Stegmaier, Philip...