In general, transcription factors have two distinct functional activities. A DNA binding activity referred to as its ability to bind the specific DNA sequence present in the regulatory element of genes is responsible for directing TFs to the vicinity of the promoter. A transactivating potential is ...
Usually, the DNA-binding domain forms a module that can be separated from the rest of the transcription factor structure without losing activity. This facilitates structural studies of the isolated DNA-binding domains or their complexes with DNA using techniques that require low molecular weight ...
DNA binding activity of transcription factor AP-1, and suppression of in vivo transcription driven by AP-1 promoter (known to be involved in breast ... L Adam,R Vadlamudi,M Mandal,... - 《Journal of Biological Chemistry》 被引量: 370发表: 2000年 A mechanism of AP-1 suppression through...
These studies indicate that the COUP box transcription factor interacts with specific base residues in the major groove of the DNA helix. In addition, we have purlfied the S300-II factor over 100,000-fold. The polypeptide possessing functional transcriptional activity has been identified by SDS-...
NFATp is one member of a family of transcriptional activators whose nuclear accumulation and hence transcriptional activity is regulated in mammalian cells. Human NFATp exists as a phosphoprotein in the cytoplasm of naive T cells. Upon antigen stimulatio
3. Wasmuth, E.V., Hoover, E.A., Antar, A., Klinge, S., Chen, Y., and Sawyers, C.L. (2020). Modulation of androgen receptor DNA binding activity through direct interaction with the ETS transcription factor ...
8. Kelley, D. R. et al. Sequential regulatory activity prediction across chromosomes with convolutional neural networks.Genome Res. (2018). doi:10.1101/gr.227819.117 9. Avsec, Z. et al. Base-resolution models of transc...
The nuclear factor B (NF-B) transcription factor family regulates genes involved in cell proliferation and inflammation. The promoters of these genes often contain NF-B-binding sites (B sites) arranged in tandem. How NF-B activates transcription through these multiple sites is incompletely understood...
The first DNA-binding activity (TRAC) is highly specific for the 8-bp element TRA and the related Escherichia coli cAMP receptor binding site. This motif is homologous to the phorbol 12-tetradecanoate 13-acetate- and cAMP-responsive elements of eukaryotic genes and the regulatory proximal sequence...
We next tested whether sites I and II synergize in activating RelA-mediated transcription. Mutation of either site, which are both weak-binding κB sites, reduced the reporter activity to near background levels, suggesting their functional cooperativity in cells (Fig. 3A, see gray and striped ...