据介绍,各种转录因子间的相互作用对组织特异性的基因表达具有重要的调控作用。为了绘制一个转录因子作用谱,科学家们分析了在人与小鼠体内起主要作用的DNA结合转录因子(DNA-binding transcription factors,TFs)。 在完成的图谱中,有762种人类的转录因子和877种小鼠的转录因子,它们可相互作用。分析研究发现,大部分的转录因...
Unbound transcription factor is then removed or washed from the solid substrate and labelled, sequence-specifically bound transcription factor is detected. Incubates which include candidate agents which alter transcription factor binding deviate from control incubates in terms of label signal - typically, ...
转录因子DNA结合域(Transcription Factor DNA Binding Domain)。 转录因子是一类能够结合到DNA上的蛋白质,它们在基因转录调控中起着至关重要的作用。转录因子通过其DNA结合域与特定的DNA序列结合,从而调控基因的转录活性。DNA结合域是转录因子中的一个重要结构域,它决定了转录因子与DNA结合的特异性和亲和力。 转录因子的...
文献解读|人类转录因子的DNA结合特异性 英文名:DNA-Binding Specificities of Human Transcription Factors 中文名:人类转录因子的DNA结合特异性 作者:Arttu Jolma, Jian Yan, Thomas Whitington, Jarkko Toivonen, et al. Jussi Taipale 第一单位:Genome-Scale Biology...
Segment 1: Sequence-Specific DNA Binding Transcription Factors序列特异性DNA结合转录因子 为确定哪部分蛋白发挥作用,将蛋白依次砍掉,若砍掉后不再与DNA结合了,说明从N端开始的第四条的蛋白序列并没有发挥作用;从C端开始的第六条蛋白序列也没有发挥作用,所以发挥作用的是最下面的部分。
Although the proteins that read the gene regulatory code, transcription factors (TFs), have been largely identified, it is not well known which sequences TFs can recognize. We have analyzed the sequence-specific binding of human TFs using high-throughput SELEX and ChIP sequencing. A total of 83...
DNA Binding Motif FAMILIES OF DNA BINDING TRANSCRIPTION FACTORS David S.Eukaryotic Transcription Factors (Fifth edition), 2008 Publisher Summary This chapter focuses on a number of differentDNA binding motifs, common to several different transcription factors, which can mediate DNA binding. It is also...
3. Morgunova, E., and Taipale, J. (2017). Structural perspective of cooperative transcription factor binding. Curr. Opin. Struct. Biol. 47, 1–8.4. Jolma, A., Yin, Y., Nitta, K.R., Dave, K., Popov, A., Taipale, M., Enge, M., Kivioja, T., Morgunova, E., and Tai...
10.3 Transcription Activating Domains in DNA Binding Proteins 10.4 Targets of Transcription Activating Factors 10.5 Examples of Repressive Transcription 10.6 Gene Silencing 10.7 Gene Silencing Example 1 10.8 Barriers to Gene Silencing 10.9 Gene Silencing Example 2 11.1 Introduction 11.2 Introduction to Transpo...
Transcription factors (TFs) bind to different parts of the genome in different types of cells, but it is usually assumed that the inherent DNA-binding pref... Aseel Awdeh,Marcel Turcotte,Theodore J. Perkins - BMC Genomics 被引量: 0发表: 2024年 Gene-specific regulation by general translation...