We employed a method based on plasmid incompatibility to cure the plasmids from Y. pestis , which confirmed the efficacy of this method for curing plasmids with different types of replicons from one bacterium. Significance and Impact of the Study: There have been no reports on the curing of ...
Different from other types of plasmids, IncP1-type plasmid have very broad transfer range even to archaea and eukarya, and its analysis is applicable to both prevention of the spread of antibiotic resistance genes and gene introduction to various organisms. Credit: Kazuki Moriguchi, Hiroshima Univer...
TheGtACR1 plasmid was described in previous studies [30]. The AQP1 (NP_932766) and KCR1 [10] were synthesized by GeneArt Strings DNA Fragments (Life Technologies, Thermo Fisher Scientific), according to the published amino acid sequences. The NCR1 was synthesized according to the genomic dat...
Individual α and β tubulin genes were cloned into separate pLIB plasmids, both of which were subsequently integrated into one pBIG1 plasmid backbone (Supplementary Fig. 1) via Gibson assembly54. Briefly, forward and reverse primers flanked the gene of interest with respective extension sequences...
Amplification of whole-cell and plasmid DNAs extracted from isolates with primers specific for the bla KPC produced an amplicon confirmed to be bla KPC-2 by sequence analysis. Pulsed-field gel electrophoresis (PFGE) typing revealed that three isolates belonged to two closely related types. ...
Objective To construct the recombinant plasmid of different hypotypes of neural cell adhesion molecule genes(NCAM).Methods The total RNA was extracted from the adult mouse cerebral cortex,and the genes of NCAM-120cDNA,NCAM-140cDNA and NCAM-180cDNA were cloned by the reverse transcriptase polymerase...
However, 5 days after mice were administered 300 μg of the DNA plasmid, the serum antibody concentration could only increase to 10 μg/ml35. After the AVV9 vector was intranasally administered to mice, the concentrations of the antibody in the nose and lung were 0.5 and 2.0 μg/...
What are the different types of enzyme inhibitors? What are enzymes and what are their functions in the cell? How many bands are formed when a plasmid is digested with one or several restriction enzymes? What are the different enzymes used in the pancreas d...
Our previous studies have shown that the inoculation of plasmid containing JEV prM-E genes could elicit considerable immune responses and protection against JEV infection in mice and pigs (Li et al. 2013a; Sheng et al. 2016). In this study, we examined whether the same DNA vaccine (pCAG-...
The cells were transfected with plasmid DNAs (1.0 µg each) using Lipofectamine 2000 reagent (11668-019; Thermo Fisher Scientific). After washing with Hanks’ Balanced Salt Solution (HBSS) (14,025,076; Thermo Fisher Scientific), occasionally, the cells were fixed with 4% PFA (P6148; ...