Multiple plasmids in different toxigenic types of Clostridium perfringens. FEMS Microbiol. Lett. 4:323-326.Duncan, C. L., E. A. Rokos, C. M. Christenson, and J. I. Rood. 1978. Multiple plasmids in different toxigenic types of Clostridi- ium perfringens: possible control of beta-toxin ...
In brief, for each dish, a combination of plasmids (listed below, optimized to ensure equal expression of Dsh and Kel constructs across multiple samples) and Lipofectamine 2000 (6 μL) were diluted respectively in 300 μL serum-free DMEM and then mixed. The resulting mixture was incubated at...
The main virulence factors are encoded on two plasmids. One produces an antiphagocytic polypeptide capsule. The other contains genes for the synthesis of three proteins it secretes: protective antigen, edema factor, and lethal factor. The combination of protective antigen with lethal factor or edema...
It has been described previously that this cluster has been horizontally transferred through some large plasmids in Alcaligenes (now Ralstonia) eutrophus H16 and A. hydrogenophilus (Lenz and Friedrich, 1998). Interestingly, a cluster of conserved The ISME Journal Comparative genomics of Alteromonas ...
A class 1 integron (dfrA12, orfF and aadA2) was detected in most of the type I plasmids. Type I contained all the plasmids carrying the blaCMY-2 gene and a subset of plasmids lacking blaCMY-2. Type II included all of the remaining blaCMY-2-negative plasmids. A sequence comparison of...
The colicin regions on both recombinant plasmids were identical with regard to restriction endonuclease sites and the arrangement of the genes. They map close to each other in the order cmi cma cbi cba , where cmi denotes the locus ... T Olschlger,E Schramm,V Braun - 《Molecular & General...
(D–F) Three sections from an E14 telencephalon electroporated with PBXW-sfGFP and CDV-hyPBase plasmids, labeled with a riboprobe for sfGFP. (G–I) EMX1-labeled sections adjacent to the above sfGFP sections. Descendants of electroporated cells, identified by sfGFP staining, are confined to ...
2014. Characterisation of blaTEM-135 genes and types of -lactamase plasmids in Neisseria gonorrhoeae--the prevalent and conserved blaTEM-135 has not recently evolved and existed in the Toronto plasmid from the origin. BMC Infect Dis 14:454 - 460. http://dx.doi.org/10.1186/1471-2334-14-...
Each plasmid sequence can be cloned in the same or other plasmids. Depending on the method of inserting desired genes into the plant, other DNA sequences may be necessary. If, for example, the Ti or Ri plasmid is used for the transformation of the plant cell, then at least the right ...
followed by a final incubation at 72° C. for 60 seconds. Sequencing of the insert of each of these three plasmids revealed that the L I-, L II-, and L III-specific amplicons were each 426 nucleotides long, and had identical nucleotide sequences, i.e., the nucleotide sequence of SEQ...