Characterization and immunological activity of different forms of recombinant secreted Hc of botulinum neurotoxin serotype B products expressed in yeast Bo Liu*, DanYang Shi*, ShaoHong Chang*, Xin Gong, YunZhou Yu, ZhiWei Sun & Jun Wu Beijing Institute of Biotechnology, Beijing, China 100071. The...
An anion-exchange high performance liquid chromatographic (HPLC) method was developed to determine the proportion of plasmid DNA in both opened and supercoiled forms. 采用阴离子交换高效液相色谱法监测重组质粒pUDKH的生产过程和该质粒产物中超螺旋pUDKH的比例。 2. To detect the effect of different propo...
PUC and CIS are the two main forms of early bladder cancer with distinct clinical, pathological, and molecular features39. According to histological grading, CIS is a high-grade tumor, while PUC could be classified into high-grade and low-grade tumors (HGPC and LGPC) with divergent molecular...
There were three types of RNases differing from each other in their intracellular localization; the enzymes in a soluble form and two bound forms associated with unknown, small particles or ribosomes and with the microsomal membrane. The top region showed a high activity per DNA content of ...
Due to a high degree of similarity between APOL proteins, it is often assumed that they have similar functions to APOL1, which forms cation channels in planar lipid bilayers and membranes resulting in cytolytic activity. However, the channel properties of the remaining APOL proteins have not been...
Here we constructed an optimized DNA vaccine plasmid containing built‐in CpG adjuvant, and designed vaccine candidates encoding five forms of antigens derived from Wuhan‐Hu‐1. The results showed that plasmid with receptor binding domain (RBD) dimer‐Fc fusing antigen (2RBD‐Fc) induced the ...
These results suggest that fragments derived from endogenous TDP43 could be produced in SH-SY5Y cells upon chronic exposure to different forms of stressors, each resulting in a unique banding pattern. Acute Stress does not Cause an Increase in ThS Signal ...
The full ectodomain of the AMA1 allelic forms from P. falciparum strains FVO, HB3, 3D7 and CAMP, as well as the in silico-designed Di versity Co vering antigens (DiCo 1, DiCo 2 and DiCo 3) [24], were expressed as recombinant proteins in Pichia pastoris. The three DiCo proteins were...
Validation of three forms of mRNA expressing RBD of SARS-CoV-2 We generated three forms of mRNA encoding the RBD of SARS-CoV-2 virus (Supplementary Fig.1A) with the aim to define the best-in-class when transfected in cultured cells. The kinetics of expression of RBD protein from 293T ce...
[17]. Each monomer forms an individual water-conducting pore, and they are further organized as tetramers in the cell membrane [9]. Studies on the tetramer structure of AQP1 have revealed that the hydrophobic helices are positioned on the outside face of the tetramer, while the hydrophilic ...