PUC and CIS are the two main forms of early bladder cancer with distinct clinical, pathological, and molecular features39. According to histological grading, CIS is a high-grade tumor, while PUC could be classified into high-grade and low-grade tumors (HGPC and LGPC) with divergent molecular...
We generated three forms of mRNA encoding the RBD of SARS-CoV-2 virus (Supplementary Fig.1A) with the aim to define the best-in-class when transfected in cultured cells. The kinetics of expression of RBD protein from 293T cells transfected with the three forms of lipofectamine-treated RBDepi...
The gel filtration profile shows Gal-13 could form a dimer even in the presence of 10 mM DTT. (F) Native PAGE of Gal-1, Gal-13 and C136S/C138S. Wild-type Gal-13 mainly forms dimers, however, it could also exist as a monomer. The double-mutant C136S/C138S exists only as a ...
To assay the presence of the precursor and mature forms of B. amyloliquefaciensα-amylase in B. subtilis, cells were separated from the growth medium by centrifugation (5 min, 12,000 rpm, room temperature), and samples for SDS-PAGE were prepared as described in Ref. 42. The B. ...
Based on a phylogenetic analysis of the VP60 gene, RHDV2 forms a separate branch, which may indicate that RHDV2 is a new number of the Lagovirus genus17. To date, RHDV2 has been detected in Spain, France, Great Britain, and Italy15,20,21,22. At present, RHDV2 has not been ...
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Because ÁSL1 deletion shortened the amplified product by 34 nt, wt and mutant forms of each RNA species were distinct. excluded from all mutant virions. In ÁSL1 virions, a strong increase of all spliced RNA species was observed, with a maximum effect for the env-...
Although purified α-IPMS-2CR was composed of both dimeric and tetrameric forms, the majority of the protein is in present as a dimer. In addition, the enzymatic activity of the dimeric form was three times higher than that of the tetrameric protein (data not shown). The majority of ...
Geminiviruses have genomes composed of single-stranded DNA molecules and encode a rolling-circle replication (RCR) initiation protein (“Rep”),
In wheat germ two forms of eIF4E of 26 and 28 kDa are present (and the gene encoding p28 is duplicated), but these two proteins share only 50% amino acid identity and are found in different cap-binding complexes (Browning et al., 1987; Allen et al., 1992; Metz et al., 1992). ...