We employed a method based on plasmid incompatibility to cure the plasmids from Y. pestis , which confirmed the efficacy of this method for curing plasmids with different types of replicons from one bacterium. Significance and Impact of the Study: There have been no reports on the curing of ...
Pulsed-field gel electrophoresis (PFGE) typing revealed that three isolates belonged to two closely related types. Plasmids electrophoresis and restriction analysis revealed that the bla KPC-2 was located on different plasmids. The transfer of carbapenem resistance from the three original isolates to ...
Plasmids were delivered by intradermal injection into the tails of mice and the types of responses generated were clearly affected by the expressed antigen. Plasmids expressing glycoproteins B and D of BHV-1, and the haemagglutinin/ neuraminidase of bPI3, generated responses with a predominance of ...
21 plasmid replicon types were detected in this experiment, with the highest prevalence of IncFII, IncFIB, IncR, and IncFIA. Notably, the detection rate of IncX3 plasmids in CROs is higher, which is different in strains with other antimicrobial phenotypes. The number of plasmid replicons they...
The cloned pGEMHE plasmids were linearized by NheIdigestion and then used as the template for in vitro RNA synthesis. cRNAs were generated using the AmpliCap-MaxT7 High Yield Message Maker Kit (Epicentre Biotechnologies) by 3-h reaction in a 37 °C incubator. Different types of cRNAs were...
Individual α and β tubulin genes were cloned into separate pLIB plasmids, both of which were subsequently integrated into one pBIG1 plasmid backbone (Supplementary Fig. 1) via Gibson assembly54. Briefly, forward and reverse primers flanked the gene of interest with respective extension sequences...
The incidence of the tpeL toxin gene was lower than that of the β2 gene. Phenotypic resistance to tetracycline was found in more than 50% of the porcine, bovine, and canine isolates, which carried a wide range of plasmids of 2–100 kb size, most of which had the tcpH clostridial ...
不同类型的并购 不同程度的整合(Different types of m a integration) 热度: Four Different Types of Writing Styles:四种不同类型的写作风格 热度: PreviewQuestions Describeatypicalprokaryotecell. Describeatypicaleukaryotecell. Explainthedifferencesbetweenaprokaryoteandeukaryotecell. ...
Analysis of the Neurotoxin Complex Genes in Clostridium botulinum A1-A4 and B1 Strains: BoNT/A3, /Ba4 and /B1 Clusters Are Located within Plasmids within the BoNT is used to categorize the species into seven immunologically distinct BoNT serotypes (A-G) which are further divided into subtypes...
Methods Construction of recombinant plasmids. For the inducible expression of BHc in P. pastoris (Table 4), four recombinant plasmids were constructed by cloning the BHc or mBHc genes into pPICZaA (Invitrogen, Carlsbad, CA, USA) or pPICZaA-pre (a pre-peptide instead of the pre-pro-peptide) ...