Integration of phage DNA with a bacterial chromosome is not the only form of lysogeny. There is a growing number of reports concerning prophages that exist in cells as plasmids. A large prophage P1...doi:10.1007/978-3-319-40598-8_54-1Magorzata obocka...
A new example of physical linkage between Tn1 and Tn21: the antibiotic multiple-resistance region of plasmid pCFF04 encoding extended-spectrum β-lactamase... A new example of physical linkage between Tn 1 and Tn 21 : the antibiotic multiple-resistance region of plasmid pCFF04 encoding extended-...
aWhen it cannot, then simply cloning the gene into a plasmid, which is subsequently grown in a non-modifying host and re-sequenced, can provide the match [9].Moreover, SMRT sequencing has also been able to identify RNA base modifications through the same approach as DNA base modificat...
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Ct values of 10 HKG in each cell line were directly related. In addition, PAR1 mRNA expression was established on LNCaP cell line using a dedicated standard curve based on serial dilutions (10-fold) of known concentrations of PAR1 full-length transcript cloned into pCR2.1 plasmid (109 to ...
aThe SEP1 sequence (nucleotides 1590–1705) and its reverse complement sequence (rSEP1) were inserted into the HindIII and KpnI sites of pcDNA3 plasmid to make the constructs used for in vitro transcription and RNP IP. SEP1序列 (核苷酸1590-1705) 和它的反向补全序列 (rSEP1) 被插入入pcDNA3...
Gill DR et al. Increased persistence of lung gene expression using plasmids containing the ubiquitin C or elongation factor 1alpha promoter.Gene Therapy2001;8: 1539–1546. ArticleCASPubMedGoogle Scholar Yew NS et al. High and sustained transgene expressionin vivofrom plasmid vectors containing a hy...
When thinking about what influences the optimal expression of a target protein, for example, you’ll have to choose between dozens of factors, like variations in the genetic payload—plasmid type, the coding, promoter or terminator sequences. The molecular biology techniques used to assemble and ...
We report the complete nucleotide sequence of a plasmid carrying the multiresistance gene cfr. This plasmid was isolated from an Escherichia coli strain of... Jian,Sun,Hui,... - 《Antimicrobial Agents & Chemotherapy》 被引量: 15发表: 2014年 Enhanced Sensitivity for Detection of HIV-1 p24 Ant...
The shRNA cassette of SUMO-1 (5'-CACCCAACACATCTCAAGAAACTCACGAATGAGTTTCTTGAGATGTGTTG-3') was inserted into pLenti4-T/O-shRNA plasmid and introduced into TREx-F3H3-K-Rta BCBL-1 cells by lentiviral transduction. Cells were selected for 14 days by 300 μg/ml zeocine (InvivoGen, ant...