plasmid-mediated AmpC β-lactamasesextended-spectrum β-lactamasesdetectionMolecular assaysIn Gram-negative pathogens, β-lactamase production remains the most important contributing factor to β- lactam resistance. β-lactamases are bacterial enzymes that inactivate β-lactam antibiotics by hydrolysis, which...
Plasmids transfer from one bacterium — the donor — to another — the recipient. A particular kind of plasmid, called IncP1, can be hosted by a variety of bacteria and, seemingly as a result of its broad hosts, can transfer DNA to recipients beyond bacteria. The hypothesis is that the ...
Individual α and β tubulin genes were cloned into separate pLIB plasmids, both of which were subsequently integrated into one pBIG1 plasmid backbone (Supplementary Fig. 1) via Gibson assembly54. Briefly, forward and reverse primers flanked the gene of interest with respective extension sequences...
PCR cloning makes it possible to breed new strains of bacteria with altered genetic makeup via the insertion of amplified dsDNA fragments into the vectors such as gDNA, cDNA, and plasmid DNA. Cloning assists in introducing point mutations using site-directed mutagenesis which itself employs the re...
Bacterial DNA ligases are NAD+dependent ligases. They are also present in some mimivirus and entomovirus. While some bacteria, archaea, and viruses, DNA ligases are both NAD+ and ATP dependent. Mycobacterium tuberculosiscodes for NAD+ dependent ligases and at least three different types of ATP-...
The syntaxin SYP132 contributes to plant resistance against bacteria and secretion of pathogenesis-related protein 1. Proc. Natl Acad. Sci. USA 104, 11850–11855 (2007). Article ADS CAS PubMed Central PubMed Google Scholar Kwon, C. Co-option of a default secretory pathway for plant immune ...
or its regression may be influenced by additional factors, according to this fact. The complex cervical milieu contains immune cells as well as a unique microbiome that controls regional immune responses [11,12,13]. Based on the types of bacteria present, the cervicovaginal microbiota was ...
In five cases out of seven the microcin-producing ability has been attributed to plasmids that the strains harboured. The effect of microcins on sensitive cells was shown to depend on ompR and ompF gene products. 展开 关键词: Microcin Peptide antibiotics Escherichia coli Plasmid determining ...
Chromosomal AmpC β-lactamases are usually inducible, while, except for DHA enzymes, plasmid-mediated AmpC enzymes are not6,7. As reported for the AmpC enzymes present in other members of the family Enterobacteriaceae expression of “AmpC-type” enzymes in Y. enterocolitica is mainly regulated ...
The resis- tant and mucoid material synthesis may also be regulated by the environment in which the bacteria grow (5,9,10). The asso- ciation of a specific plasmid with the presence of mucoid phe- notype was not found in our isolates because both mucoid and nonmucoid strains of the two...