The invention is a method for isolating a desired plasmid DNA isoform, typically a supercoiled isoform, which includes the steps of obtaining a quantity of bioreactor produced plasmid DNA in liquid form for purification, and passing the plasmid DNA through an ultrafiltration membrane at a selected ...
In most instances the mixing of plasmids had only slight effects on the magnitude or bias of the responses to the individual components. However, under certain conditions we found that addition of a second plasmid converted an IgG2a biased response to a response with primarily IgG1 antibody. The...
Plasmids are extrachromosomal fragments of DNA that enable rapid adaptation and evolution by transferring genes conferring selective advantages to their hosts. Notably, the term “replicon” can be used to describe any DNA segment that can self-replicate, such as a plasmid. Alternatively, it can ref...
Both the cell lines were liposome-transfected by pGFP-EB1 plasmid28 (Addgene 17234) by means of the torpedo DNA transfection vector (Ibidi) in DMEM media without sera and antibiotics according to man- ufacturer's protocol. Live cell microscopy started 18–48 hrs after transfection. Live ...
geneticinformationisinacircularloopcalledaplasmid(insteadofhavingchromosomalDNA) Strongcellwalls:resistanttoenvironmentalchanges Size Bacterialcellsareverysmall, about1-2µmindiameterand10µmlong Onemicrometer=1/1000mm. Imaginelookingatadimefromitsthinnestsideandtryingtosliceitintoathousandpieces;thatisthesame...
However, 5 days after mice were administered 300 μg of the DNA plasmid, the serum antibody concentration could only increase to 10 μg/ml35. After the AVV9 vector was intranasally administered to mice, the concentrations of the antibody in the nose and lung were 0.5 and 2.0 μg/...
Evaluation of Maltose-Based Cationic Liposomes with Different Hydrophobic Tails for Plasmid DNA Delivery 来自 mdpi.com 喜欢 0 阅读量: 62 作者:B Li,L Deng,M Liu,Y Zeng 摘要: In this paper, three cationic glycolipids with different hydrophobic chains Malt-DiC12MA ( IX a ), Malt-DiC14MA (...
Protection against lethal Rift Valley fever virus (RVFV) infection in transgenic IFNAR(-/-) mice induced by different DNA vaccination regimens. In this work, plasmid constructs encoding two different M segment ORFs, as well as the nucleoprotein N, have been used in different vaccination regimes ...
Individual α and β tubulin genes were cloned into separate pLIB plasmids, both of which were subsequently integrated into one pBIG1 plasmid backbone (Supplementary Fig. 1) via Gibson assembly54. Briefly, forward and reverse primers flanked the gene of interest with respective extension sequences...
The cells were transfected with plasmid DNAs (1.0 µg each) using Lipofectamine 2000 reagent (11668-019; Thermo Fisher Scientific). After washing with Hanks’ Balanced Salt Solution (HBSS) (14,025,076; Thermo Fisher Scientific), occasionally, the cells were fixed with 4% PFA (P6148; ...