CTAB Total DNA IsolationCTAB Total DNA IsolationDoyle, JeffreyDoyle J: DNA protocols for plants - CTAB total DNA isolation. In Molecular Techniques in Taxonomy. Edited by Hewitt GM, Johnston A. Berlin: Springer; 1991:283-293.Doyle J: DNA protocols for plants-CTAB total DNA isolation. In Mole...
DNA Protocols for Plants DNA protocols for plants: CTAB total DNA isolation . In: Hewitt GM , Johnston A eds. Molecular techniques in taxonomy . Berlin : Springer-Verlag. 283... J Doyle - Springer Berlin Heidelberg 被引量: 588发表: 1991年 ...
Total genomic DNA was extracted from samples collected from symptomatic (10 plants) and asymptomatic healthy-looking plants (10 plants) using a CTAB method and amplified with universal primers P1/P7 and R16F2n/R16R2 in direct and ... Mürsel ATAL,C Ikten,E Yol,... - 《Plant Disease》 ...
DNANucleicacidCTABSilicaPlantvirusPlantpathogenTo assay for viruses in plant samples, we required a method for nucleic acid isolation that is rapid, simple, and applicable to the widest possible variety of plants. A protocol for isolation of total nucleic acid (TNA) was developed by combining ...
CTAB结合DNA凝胶回收试剂盒提取食用菌DNA ３２ 生物技术 且污染较少［Ｉ引。综合前人研究成果，作者采用间接法提取青贮饲料中的微生物总ＤＮＡ，使用了ＣＴＡＢ试剂，采用异丙醇沉淀ＤＮＡ。吸光度和ＰｃＲ检测是评价ＤＮＡ质量常用的手段。临１，本研究中通过光吸收、琼脂糖电泳及ＰｃＲ检验，结果表明提取的ＤＮ...
2.Method: The improved CTAB method and improved SDS method were used to extract DNA.方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。 3.By studying the appearance,concentration,A260/A280,A260/A230 and electrophoresis,and comparing the total DNA extracted...
关键词:甘薯块根;CTAB法;RNA 中图分类号:Q522 文献标识号:A 文章编号:1001-4942(2008)09—0090—03 ImprovedCTAB—LiClSystemforTotalRNAIsolationfrom TuberousRootsofSweetPotato HOUFu—yun。ZHAOBing,ZHAOBao—jie,WANGQing—mei,LIAi—xian, ZHANGHai—yan,DONGShun—XU,ZHANGLi—ming (1.CropResearchInstitute...
MethodsAn improved CTAB method was presented here to be suitable for isolation of total RNA from Rhododendron leaf , a plant which contained abundant secondary metabolites. Using chemical CTAB as detergent in the extraction buffer, the absolute ethanol precipitation of RNA was performed finally after ...
结论该方法对杜鹃花等富含次生代谢产物的植物特别是富含多酚、多糖的材料的总RNA提取具有借鉴意义关键字:RNA提取;杜鹃花;改良CTAB法ChinaAbstract:ObjectiveTostudythemethodforisolationoftotalRNAfromRhododendronleaf.MethodsAnimprovedCTABmethodwaspresentedheretobesuitableforisolationoftotalRNAfromRhododendronleaf,aplantwhich...
BasedontheCTABextractionmethodforcottontotalDNAandtheLiClprecipitationmethodofRNA,anewmethodsuitableforcottontotalRNAextraction,namedCTAB2acidicpheon2licmethod,wascomeintobeing.ThemethodcouldproducecottontotalRNAwithhigherpurityandintegralityinashortertimefromdifferenttis2suesofcottonthanotherconventionalmethods.Keywords:...