CTAB method.Isolation of good quality and quantity of DNA is essential for the purpose as well as for preserving the same to a considerable period of time. Although the DNA needed per reaction in PCR based makers is very low, the number of PCR reactions to be performed is large and hence...
11. 每个样品用2050 L TE溶解DNA,并添加12 L无DNase的RNase A(10 mg/mL)。37°C孵育30分钟。 12. 确定浓度后,贮存于4°C(或长期贮存于20°C)。Small-scale Preparation of Plant Genomic DNA Using CTABPrinciple The CTAB method of DNA isolation was initially used in bacteria and 6、later ...
DNA Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method 利用CTAB法提取蝴蝶兰的基因组 www.ilib.cn 2. DNA Isolation of genomic DNA from different organs in Anthurium andraeanum by CTAB method 利用CTAB法提取红掌不同部位基因组 www.ilib.cn 3. DNA extraction of Staphylococcus aureus using th...
This paper extracts the genomic DNA of the young leaves of pea(Pisum sativum L.)by using an improved CTAB method,and makes the ultraviolet spectrophotometer and agarose gel electrophoresis.The results show that the obtained DNA,which presents better primary structure,higher purity and concentration,co...
An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves改良CTAB法用于多年生植物组织基因组DNA的大量提取* An Efficient Macro-metho... It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharides, such as ...
High quality genomic DNA ca n be isolated from Actinidia plant using th e improved method. The DNA is good e noug h for Southernblot and other u sesin DNA research. The protocol is also efficient for qu ick and macro DN A extraction.Key words:Actinidia ; genomic DNA; isolation; ...
Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue This report summarizes major changes in previously published protocols for DNA extraction to improve the quality of DNA extracted from plants. Here, we hig... JH Cota-Sánchez,K Remarchuk,...
Isolation of plant DNA fresh tissue JJT Doyle,JL Doyle 被引量: 2228发表: 1990年 A quick and inexpensive method for removing polysaccharides from plant genomic DNA. A quick and inexpensive method has been demonstrated to remove polysaccharide contamination from plant DNA. Isolated plant genomic DNA...
真菌DNA小量提取试剂盒(CTAB和离心柱法) (A-DH854)真菌DNA小量提取试剂盒(CTAB和离心柱法) 货号:A-DH854 Solar® HP Fungal DNA Mini Kit 背景资料:Isolation of plant DNA with a combination of the CTAB method and spin column technology. The Solar® HP Fungal DNA Kit is designed for ...
ctabdna蒜黄法提取花苗缓冲液 中央民族大学生命与环境科学学院生物化学综合性设计实验报告2011年12月17日姓名:***:1043050年级:2010级专业:生物科学小组成员:张文浩,覃亚,马福秀指导教师:**亚CTAB法提取大蒜黄化苗中的DNAIsolationofDNAintheyellowseedingofgarlicbyCTABmethodCTAB法提取大蒜苗中的DNA李真,张文浩,覃亚,...