We present a simple protocol for DNA isolation from climbing cacti, genera Hylocereus and Selenicereus. The abundant polysaccharides present in ttylocereus and Selenicereus species interfere with DNA isolation, and DNA extracts, rich in polysaccharides, are poor templates for amplification using polymerase...
Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components A relatively quick, inexpensive and consistent protocol for extraction of DNA from expanded leaf material containing large quantities of polyphenols, tanni......
Isolation of high quality DNA from multiple samples can be both time-consuming and expensive. We have developed a combined protocol to reduce the time comp... Fu,ZY,Song,... - 《Journal of Integrative Agriculture》 被引量: 1发表: 2017年 Molecular identification of ascochyta blight of Cicer ...
The protocol is also efficient for qu ick and macro DN A extraction.Key words:Actinidia ; genomic DNA; isolation; Southern blot DNA 保存方式包括 DNA 分子保存( 即 DNA 库保存) 、 基因组文库保存、 cDNA 文库保存和特殊基因片段保存等。采用 DNA 保存, 不仅具备其他资源保存方法的一般特点, 还具有...
A number of methods have been devised for DNA extraction and purification, and some are described here. First, the CTAB isolation protocol for total DNA is... BH Taylor,JR Manhart,RM Amasino 被引量: 69发表: 1993年 Population genetics of plant pathogen interactions: the example of the Erysiph...
Using a SDS-based protocol to extract DNA from several herbarium Basidiomycetes specimens, Bruns et al. (1990) successfully extracted and PCR- amplified DNA from 31 distinct species. Nevertheless, all taxa used in their work were from the order Agaricales, embrassing species with fleshy and ...
Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a variety of cytoplasmic components like polysaccharides, polyphenols, lipids, and proteins. This demands modification in the DNA extraction protocol to obtain DNA in the ...
Here we have used CTAB for DNA extraction not only from plants but various other samples like FUNGI, ALGAE, BACTERIA, and HUMAN BLOOD. In this paper, not only isolation is performed from various samples but also an optimized protocol is introduced. Same protocol is used for all the sam...
Isolation of DNACTAB method.Isolation of good quality and quantity of DNA is essential for the purpose as well as for preserving the same to a considerable period of time. Although the DNA needed per reaction in PCR based makers is very low, the number of PCR reactions to be performed is...
DNA isolationsilica columnsIsolation of high quality DNA from multiple samples can be both time-consuming and expensive. We have developed a combined protocol to reduce the time component of the hexadecyltrimethylammonium bromide(CTAB) extraction method and reduced costs by regenerating the silica ...