We have CTAB (Cetyl tri methyl ammonium bromide) method for isolation of DNA from plant tissue. The method is suitable for isolating DNA from a small to mediumnumber of plant samples. The DNA can be stored for a longer duration. The method involves extraction of DNA using a buffer (pH ...
Small-scale Preparation of Plant Genomic DNA Using CTABPrinciple The CTAB method of DNA isolation was initially used in bacteria and 6、later modified to obtain DNA from plants. CTAB (cetyltrimethylammonium bromide) is one of cationic detergents. CTAB forms a complex with nucleic acids, and ...
Talking about the Isolation of Genomic DNA from Pea with Improved CTAB Method 来自 知网 喜欢 0 阅读量: 16 作者:XH Zou,CW Liao 摘要: This paper extracts the genomic DNA of the young leaves of pea(Pisum sativum L.)by using an improved CTAB method,and makes the ultraviolet spectrophotometer ...
- 1 -CTAB 法小量制备植物基因组DNA 【原理】CTAB 分离DNA 的方法最初用于细菌，后来经修改用于从植物中获取DNA 。CTAB （即十六烷基三乙基溴化铵）是一种阳离子去污剂。CTAB 可与核酸形成复合物，该复合物仅溶于高盐溶液。此外，CTAB 可十分有效地促进DNA 和RNA 与多糖的分离并去除多糖。可通过提高NaCl 的...
PLANT DNA ISOLATION - A METHOD TO EFFICIENTLY REMOVE POLYPHENOLICS, POLYSACCHARIDES, AND RNA The procedure described below alleviates the time and expense of CTAB (cetyltrimethylammonium bromide) and caesium chloride methods by utilizing PVP (poly... Jobes,DV,Hurley,... - 《Taxon》 被引量: 222...
The method involves inactivation of contaminant proteins by using CTAB/Proteinase K and precipitation of polysaccharides in the presence of high concentration of salt. The purity of genomic DNA was confirmed by A260/280 and A260/230 ratios calculated from the spectrophotometric readings and further ...
CTAB Total DNA IsolationCTAB Total DNA IsolationDoyle, JeffreyDOYLE J. DNA protocol for plant-CTAB total DNA Isolation[A]. In: Hewitt, GM, Johnston A. (Eds.),Molecular Techniques In Taxonomy [ M ]. Berlin:Springer, 1991,283 - 293.Doyle...
A simple,rapid genetic DNA extraction procedure by improving the CTAB(cetyltrimethyl ammonium bromide) method has been established due to high quantity of phenol and polysaccharide contained in cotton,thus genetic DNA of cotton with high purity can be obtained.The optimized system and experimental prog...
产品简介:是简单快速简便的提取植物总DNA的试剂盒,先将新鲜植物样本研磨破碎细胞壁,加入CTAB抽提液使细胞膜破裂同时将核酸与植物多糖等杂质分开。.CTAB抽提液的有效成分为CTAB(十六烷基三乙基溴化铵),经蛋白清除液等去除蛋白,即可获得植物基因组DNA,可进行酶切、PCR等下游实验。备注:产品详细报价和货期,说明书请咨询子...
using quantitative real time PCR (qPCR). We compared DNA isolated from nuclei to total cellular DNA isolated with the CTAB method. As expected, DNA isolated from nuclei consistently yielded nuclear DNA with fewer chloroplast and mitochondrial DNA copies, as compared to the total cellular DNA ...