Bacterial genomic DNA isolation using CTAB, Department of Energy Joint Genome Institute, https://jgi.doe.gov/user-programs/pmo-overview/protocols-sample-preparation-information/ (2012). Arkin, A. P. et al. KBase: the United States Department of Energy systems biology knowledgebase. Nat. ...
DNA isolationPCRCTABIsolation of nucleic acids of different plant pathogens is one of the most important stages, which have influence on the correct molecular diagnosis of these pathogens. This study investigated the suitability of two different DNA isolation techniquesused in the diagnosis of ...
(CTAB)-terminatednanorods(Fig. 3a–b) [94] and poly(l-lysine)-coated Aunanoparticles(Fig. 3c–d) [95]. Nevertheless, for mildly-negatively-charged gram-negative bacterial cells, direct conjugations of the nanomaterials may conceivably lead to the easy disintegration of the nanohybrids. Therefore...
Pellet the cells by centrifugation (1 minute, 12,000 – 16,000 × g), remove the supernatant, and use the cells for gDNA isolation using a commercial kit or standard protocols [e.g. CTAB total DNA preparation (13) or phenol-chloroform extraction (14)]. Here, a commercial kit was used...
DNA samples were extracted using the modified CTAB method (Murray and Thompson 1980) and were standardized to 50 ng/μl for Illumina 384-plex BeadXpress SNP genotyping. The 380 genotypes were genotyped using the 384-plex indica x indica SNP set (VC0013033-OPA) on Illumina BeadXpress system ...
Promoter isolation Genomic DNA was extracted from fresh ‘E-32’ (S) S. melongena leaves using the DNeasy Plant Mini Kit (Qiagen, Germany). The promoter of the eggplant isochorismate synthase 1 (ICS1) gene was isolated using a Genome Walker Kit (Clontech, USA) by nested PCR (primers sho...
phaseoli genomic DNA by using the CTAB method. We found clear DNA band in 0.3% agarose gel electrophoresis. A sensitive and specific assay was developed to detect common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli in leaves of common beans. Primers Xap 27 F and Xap 1319 R...
For example, the exact role of TTSS in DNA isolation from selected fruiting bodies was Rhizobium species is unknown, but the system is performed using a purification kit for the isolation of certainly necessary in the symbiotic interaction of DNA from plant material (Promega, Madison, WI, this ...
The virulence genes may be cloned by PCR, using P. multocida genomic DNA as the template. For ease of inserting the gene into expression vectors, PCR primers are chosen so that the PCR-amplified gene has a restriction enzyme site at the 5′ end preceding the initiation codon ATG, and a...
The final product was suspended in TE buffer and the DNA concentration was determined as described in Plasmid Purification. An alternate method for preparing genomic DNA was the CTAB procedure (30): In this procedure, the cells are lysed with SDS and proteinase K, and contaminants are ...