Upon recovery of a population that hybridized both probes and analysis of 1.4 kb 16S rRNA gene sequences obtained from their amplified genomic DNA, it was found that at least four different non-Acidobacterium species hybridized the probes. Additionally, our results indicated that probe length and ...
The total genomic DNA was extracted from the soil samples using the E.N.Z.A. Soil DNA Kit (Omega Bio-tek), following the manufacturer’s protocol (http://omegabiotek.com/store/product/soil-dna-kit/). DNA quantity and quality were evaluated by spectrophotometry (NanoDrop) and agarose gel ...
All soil samples were stored at − 20 °C prior to DNA extraction and analysis. DNA extraction and sequencing DNA was extracted from 0.5 g (total humid weight) of soil using the Power Lyzer Power Soil DNA Isolation Kit (MoBIO Laboratories, Carlsbad, CA, USA), according to the ...
EnteroBaseand theCenter for Genomic Epidemiology. Nowadays, there are other alternatives for conducting WGS analysis, as more software are appearing regularly, such as the recently publishedBactopiaandASA³P. Every software offers different features towards specific outcomes, providing the users with a...
英文名Rapid Bacterial Genomic DNA Isolation Kit 相关类别基因组DNA分离储存常温(10-30℃) 编 号包装库存目录价(￥)您的价格(￥)数量 B518225-005050 PREPS现货230230 B518225-0100100 PREPS现货420420 产品描述 概述 本试剂盒是生工生物研制成功的一种细菌基因组 DNA 快速抽提试剂盒。通过水浴裂解、去蛋白、异丙醇...
These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturing of bacteria, which are known for their selectivity leading to a non-representative view of the extent of bacterial diversity. This review provides an ...
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Isolation and identification of clinical isolates from tissues of OLP lesions. (A) Aligned nucleotide sequences of the 16S rRNA gene from four clinical isolates andE. coliK-12 MG1655. (B) Phylogenic analysis of 15 strains in theE. coligroup. The numbers on the branches show the branch lengt...
Bacterial strains and genomic DNA isolation Strain RM1221 was obtained from Food Safety and Health Research Unit, USDA. Strain NCTC 11168 was obtained from the American Type Culture Collection (Mannassas, VA). Genomic DNA isolation was carried out as previously described [16] Construction of a C...
stephensi (male/female/larvae) was used for the isolation of the culturable micro flora. The bacterial colonies on TSA and LB agar were selected on the basis of minor variations using conventional microbiological techniques. The initial number of isolates was reduced based on colony characteristics ...