Genomic DNA was extracted from cultured single bacterial colonies as described12. For genome sequencing of STB-D, STB-J, STB-K and STB-L, Sanger reads from 10-kb fragment shotgun libraries at 4- to 4.9-fold coverage were assembled with contigs obtained from Newbler assemblies of 454/Roche ...
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Culture-independent isolation of environmental bacterial species for genomic analysis.Fluorescence in situ hybridization (FISH) has been a commonly used method in molecular ecology studies. In many cases, one can visualize bacterial populations based upon the hybridization of FISH probes with bacterial ...
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In brief, ~105 A549 cells were infected with ~107 bacterial CFU and incubated for 60 min at 37 °C in 5% CO2 (v/v) atmosphere. Non-adherent bacterial cells were removed by washing with PBS. Infected cells were lysed by the addi- tion of 1 ml distilled water and serial 10-...
英文名Rapid Bacterial Genomic DNA Isolation Kit 相关类别基因组DNA分离储存常温(10-30℃) 编 号包装库存目录价(￥)您的价格(￥)数量 B518225-005050 PREPS现货230230 B518225-0100100 PREPS现货420420 产品描述 概述 本试剂盒是生工生物研制成功的一种细菌基因组 DNA 快速抽提试剂盒。通过水浴裂解、去蛋白、异丙醇...
Citrus industry is affected worldwide by several fungal, bacterial and viral diseases. Some production areas also suffer of strong abiotic constraints such... O Patrick 被引量: 0发表: 2013年 Abiotic Stress and Hormonal Responses of a Promoter of Citrus Carotenoid Isomerase Gene in Transgenic Arabid...
2.1. Cell culture 293T cells were maintained in DMEM (Wako) supplemented with 10% fetal calf serum (FCS). 2.2. Plasmids The plasmid encoding hCas9 D10A was purchased from Addgene (#41816) [22]. To construct dCas9, the H840A mutation was introduced into hCas9 D10A with QuickChange ...
Bacterial culture, genomic DNA preparation, next-generation sequencing (NGS),de novogenome assembly and merging of contigs and subsequent bioinformatics analyses workflow are explained in detail below. Figure1presents a flow chart for the complete workflow. ...
att sites direct site-specific integration and recombination of phage DNA into the bacterial genome. Of the 9 putative intact prophages that we found, 6 prophages are present in more than one genome and 3 prophages are strain-specific (Supplementary Fig. S8). The 6 shared prophages contributed ...