DNAThe performance of different anion-exchange media have been compared for the isolation of plasmid DNA and genomic DNA from bacterial cells and human whole blood. Whatman DEAE-Magarose, based on an agarose bead containing a paramagnetic component, has been compared with prepacked gravity-flow ...
Because HuABCB6 was overexpressed in human cancers compared to peri-tumoral tissue in the absence of gene amplification, transcriptional regulation may play an important role in its expression. Therefore, we isolated a 14 kb genomic DNA clone containing the HuABCB6 promoter and 5′-flanking region...
Newcellulasegene isolationfromthermophilic bacteriais a promising alternative in biotechnological, environmental, and industrial applications. Currently, several approaches, such as genomic,metagenomic(direct and substrate enriched), and bioinformatics methods, used toward obtaining a new gene from thermophilic ...
A method for isolating high quality DNA from wholeEuglena cells is described. The procedure consists in: the weakening of the cell pellicle in glycerol avoiding the mechanical disruption of cells and shearing damage in DNA molecules; the decondensation ofEuglena compact chromatin directly inside the ...
Strains that did not agglutinate with any of the sera were regarded as non-typable (NT). Triplex PCR, HRM and sequencing Genomic DNA was isolated with the Genomic Mini kit (A&A Biotechnology, Gdynia) for isolating genomic DNA from bacteria, cell cultures and solid tissues. DNA was ...
Plasmid DNA (pDNA) isolation, also known as plasmid purification, is the process of extracting and purifying pDNA from bacterial cells, allowing scientists to obtain a highly concentrated and pure form of the plasmid for further analysis and experimentation. ...
Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure. Although several methods for isolating genomic DNA from trypanosomatid protozoa exist [1鈥 3], all are time-consuming and cumbersome. Faster, simpler and ef... E Medina-Acosta,GAM Cross - 《Molecular & Bi...
Eukaryotic cells (max. 5 × 106) GenUP™ Blood DNA Kit Isolation of genomic DNA from whole blood Fresh or frozen whole blood; stabilized with EDTA or citrate (200 µl or 400 µl) GenUP™ Plant DNA Kit Plant DNA isolation from various materials Fresh, frozen or dry plant material...
The COVID-19 pandemic has led people to wear face masks daily in public. Although the effectiveness of face masks against viral transmission has been extensively studied, there have been few reports on potential hygiene issues due to bacteria and fungi a
Separation of bacterial DNA from human DNA in clinical samples may have an important impact on downstream applications, involving microbial diagnostic systems. We evaluated two commercially available reagents (MolYsis®, Molzym GmbH & Co. KG, Bremen and Pureprove®, SIRS-Lab GmbH, Jena, both Ge...