We have successfully adapted the Wizard(R) MagneSilTM Plasmid DNA Purification System (Cat.# A1635) to isolate genomic DNA from soil bacteria important in research and the agricultural industry. In this article, we describe the modified protocol for isolating genomic DNAfrom ...
We present a protocol for the preparation of DNA suitable for genomic studies, starting from uncultured intracellular bacteria. The study was conducted on Midichloria mitochondrii , the intramitochondrial symbiont of the tick Ixodes ricinus . Single oocytes, which carry a high load of symbionts, ...
To better understand how microalgae bacteria work together to remove nutrients from wastewater, functional genomics helps study technical aspects of microalgae-bacterial co-cultures (Perera et al., 2019). New insights regarding the metabolic engineering of microalgae that can remove antibiotics are ...
Genomic DNA (gDNA) was isolated from pelleted bacteria using the Roche High Pure PCR kit following the standard protocol for isolation of nucleic acids from bacteria (Roche Applied Science, Indianapolis, IN). The quality of the gDNA extracted was determined through gel electrophoresis and quantity ...
The simplified protocol consists of only three major steps, heat extraction of genomic DNA from rhizobial cells prepared from dried nodules, ethanol precipitation of the DNA and Sephadex G-50 column purification of the DNA, and generated fingerprints with good quality for differentiation of Rhizobia...
The sewage samples contain variable proportions of non-bacterial DNA, which is not relevant to ARG quantification. Since we want a measure to reflect how much resistance is carried by the bacteria in the sample, we adjusted the number of ARG-assigned fragments to a proxy for bacterial load per...
Which kit for genomic DNA extraction from bacteria is right for you? Order now Order now Order now Order now Most cost-effective Fast isolation of gDNA from bacterial cells High-yield, high-purity gDNA in a plate formatRapid and automated extraction of DNA DNAzo...
Protocol time30 mins45 mins45 mins Isolation methodSilica plateScalable, flexible format with magnetic beadsFast, convenient silica column High-throughput compatible Number of preps38448050 To the top Tissue DNA extraction from tissue Obtaining high-quality, intact DNA is oft...
Protocol time30 mins45 mins45 mins Isolation methodSilica plateScalable, flexible format with magnetic beadsFast, convenient silica column High-throughput compatible Number of preps38448050 To the top Tissue DNA extraction from tissue Obtaining high-quality, intact DNA i...
PDIM participates in the prevention of phagosomal acidification33, an important requirement for the enzymic digestion of internalized bacteria. PGL in M. tuberculosis has been reported to affect cytokine production in the host; more specifically to reduce pro-inflammatory cytokine release....