Hereby, we present the first protocol for enriching bacterial DNA from tissue biopsies that allows efficient isolation of all bacteria. Our protocol facilitates analysis of a wide spectrum of bacteria of clinical tissue samples improving their applicability for microbiome research....
This is an optimized DNA isolation protocol adapted to the properties of bacteria isolated from biogas substrate and digestate, using NucleoBond AXG Columns and NucleoBond Buffer Set. The protocol reliably retrieves DNA of sufficient quality, length and yield. The performance has been validated on ...
The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA. Here, we present a DNA isolation protocol that is suitable for a wide variety of GI tract samples, including biopsies with minute amounts of material...
The Ethics Committee of the University Hospital, Rhine-Westfalian Technical University (RWTH), approved a protocol describing the specimen collection for this investigation, and each individual signed an informed consent to participate. For the caries samples a cotton tampon was used to collect un-...
The 109 patients with STEMI received antithrombotic therapy with Acetilsalicic Acid, Clopidogrel, Ticagrelor or Prasugrel, according to the local AMI management protocol. Table 2 Baseline Characteristics of study population. Full size table Presence of bacterial DNA in thrombi from AMI patients Of the...
(EQUC) protocol and similar enhanced (metaculturomic) methods have enabled researchers to isolate species detected by 16S rRNA gene sequencing in both individuals with and without urinary tract symptoms [7,8,11,13,14,15]. While the genetic diversity of some species has been extensively ...
By the use of these methods, it has long been realized that molecular biology grade (MBG) reagents, DNA isolation kits, PCR master mixes, and several other laboratory supplies used in processing and analyses of DNA are contaminated with bacterial DNA [3–7]. Although microbial contamination ...
(Mobio Laboratories Inc., Carlsbad, CA, USA) was used according to the manufacturer's protocol. DNA quality was checked by 1% (w/v) agarose gel electrophoresis, and the amount was determined using a Qubit 2.0 fluorometer (Life Technologies: Carlsbad, CA, USA). The extracted DNA was stored...
(0.1 mm) containing 650 μl of Bead Solution. The remainder of the DNA isolation protocol was continued beginning with step 4 of the PowerLyzer PowerSoil DNA Isolation Kit instructions (MO BIO laboratories, Inc., Carlsbad, CA). The bead-beating step used a FastPrep-24 Instrument (MP ...
By the use of these methods, it has long been realized that molecular biology grade (MBG) reagents, DNA isolation kits, PCR master mixes, and several other laboratory supplies used in processing and analyses of DNA are contaminated with bacterial DNA [3–7]. Although microbial contamination ...