Isolation of DNA from seaweeds has proven difficult because of coprecipitation of polysaccharides. Our process minimizes this contamination, which is mostly due to the highly hydrocolloidal content of algal cell walls. This protocol, using 2 steps, is based on a preliminary enzymatic digestion of ...
A general procedure for purifying and efficiently separating four types of DNA from filamentous fungi has been developed. The protocol involves (i) disruption of mycelial cells by blending in liquid nitrogen followed by suspension of cell contents in buffer containing high concentrations of protease and...
DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet ...
This modified protocol used 1.6 M NaCl, 2% CTAB and 2.5% PVP of molecular weight 40,000, 0.5% β- mercaptoethanol and an incubation period of 35 min at 60 o C. The purity of the extracted DNA was assessed by spectrophotometry. The quantity of DNA obtained was equivalent to 238.5g per ...
avingedicinal properties.econdaryetabolites,ainly polyphenols,auseinderance inhe isolationf good quality DNAromhe plant.herefore, inhe present investigation,nttempt wasadeo determineheotal phenolicontents in various partsf in vitro grown plantletsnd DNA isolation wasptimizedrom in vitro grownresh ...
J. & 30 DNA extraction from bas... GN Aristóteles,LL Clarice,RT Guerrero - 《Biotemas》 被引量: 73发表: 2005年 An inexpensive method for extraction of genomic DNA from fungal mycelia A rapid and efficient protocol for the extraction of genomic DNA from plant pathogenic fungi was developed...
This method can be used on all forms of ivory. However, DNA recovery is highest when the outermost layer of the tusk, the cementum, is used. When applied to extract DNA from 11 samples, in duplicate, the entire protocol can be completed in 6 d, although much of this time consists of...
Objective To establish and appraise the technology of the fast preparation of mitocondrial DNA fragment from the micro blood. Methods improving the method ... QS Cai,N Zhang,Y Zhang,... - 《Journal of Sichuan University》 被引量: 3发表: 2002年 Optimization of a Protocol for The Extraction...
MODIFICATION OF DNA ISOLATION PROTOCOL FROM SILICA GEL DRIED-LEAF TISSUES OF Pinanga (PALMAE) 来自 e-journal.biologi.lipi.go.id 喜欢 0 阅读量: 25 作者:JR Witono,K Kondo 被引量: 4 年份: 2006 收藏 引用 批量引用 报错 分享 全部来源 免费下载 求助全文 e-journal.biologi.lipi.go.id (...
The new protocol can be successfully used for both small- and large-scale preparation of genomic DNA from different tissues of Dioscorea spp. The quarantine of seed tubers and use of pathogen-free tubers for planting is a prerequisite for integrated disease management strategy. The protocol can ...