Protocol : Isolation of Genomic DNA from Laser- Microdissected TissuesDna, QiaampKit, MicroAe, Equilibrate BufferAw, BuffersRna, CarrierAl, BufferAtl, Buffer
Protocol 切尾片(3mm)和马克的耳朵。 720,M,L,STE和30M,L,蛋白酶K(10毫克/毫升的股票) 。 孵育55°C。加热块和涡小时每3小时的最高速度为10秒 在70℃5分钟灭活蛋白酶K。 淬火冰5分钟。 离心10分钟全速尾部DNA。 倒出到新管含720米升异丙醇。
DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, no. 51306) following the manufacturer’s protocol. For selected pedigrees, RNA was isolated from either fetal lung, fetal kidney, cultured fibroblasts or parental whole blood for RNA-seq or RT–PCR analysis. RNA was extracted using the...
RNA isolation was performed as suggested in the Tuveson Laboratory Murine and Human Organoid Protocols, briefly the culture media was aspirated away, and the Matrigel domes were dissolved into TRIzol Reagent (Thermo Fisher), followed by the RNA isolation protocol recommended by the manufacturer. Librar...
The Sidewinder Genomic DNA Purification Kit provides simple, small-scale isolation of high quality genomic DNA from a wide range of animal tissues. Based on a modified salting-out procedure, the kit does not employ a chromatographic step or organic extractions. The kit uniquely includes ...
英文名:NucleoSpin DNA Trace (4) 4 preps for the isolation of genomic DNA from forensic samples Buffer FLB, B5, BW, BE Proteinase K NucleoSpin DNA Trace F columns, Elution tubes Protocol 中文名: CBNumber:CB41002724 分子式: 分子量:0 MOL File:Mol file NucleoSpin DNA Trace (4) 4 preps...
Protocol description: The Dynabeads® SILANE genomic DNA kit provides an excellent tool for automated gDNA isolation, following a simple and scalable protocol (Figure 2). A buffer is first added to the sample for lysis, followed by incubation with Dynabeads® MyOne™ SILANE. When placed in...
Taggart JB, Hynes RA, Prodöuhl PA, Ferguson A (1992) A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol 40:963–965 Article CAS Google Scholar Taylor SA, Larson EL (2019) Insights from genomes into the evolutionary importance and prevalence of hybridi...
Genomic DNA (gDNA) was isolated from pelleted bacteria using the Roche High Pure PCR kit following the standard protocol for isolation of nucleic acids from bacteria (Roche Applied Science, Indianapolis, IN). The quality of the gDNA extracted was determined through gel electrophoresis and quantity ...
151.DescriptionTheWizard®SVGenomicDNAPurificationSystem(a)providesafast,simpletechniqueforthepreparationofpurifiedandintactDNAfrommousetails,tissuesandculturedcellsinaslittleas20minutes,dependingonthenumberofsamplesprocessed.Forthissystem,eitheraspinorvacuum(“SV”)purificationprotocolcanbeused(Figure1).Upto20mg...