DNACloning, MolecularGenesWithin the scope of this laboratory guide it is impossible to give a full review of all the procedures used in the isolation of intact genomic DNA. However, we shall describe a general method we used which gives an excellent quality of both nuclei and DNA suitable ...
Within the scope of this laboratory guide it is impossible to give a full review of all the procedures used in the isolation of intact genomic DNA. However, we shall describe a general method we used which gives an excellent quality of both nuclei and DNA suitable for genomic sequencing. Whe...
This paper extracts the genomic DNA of the young leaves of pea(Pisum sativum L.)by using an improved CTAB method,and makes the ultraviolet spectrophotometer and agarose gel electrophoresis.The results show that the obtained DNA,which presents better primary structure,higher purity and concentration,co...
- 《Canadian Journal of Botany》 被引量: 200发表: 1990年 Small-scale isolation of genomic DNA from Streptomyces mycelia or spores. Kutchma,A.,M.A.Roberts,D.B.Knaebel and D.L.Crawford, Small scale isolation of genomic DNA from Streptomyces mycelia and spores. Bio Techniques 24: ... AJ ...
摘要: A simple and rapid way for isolation of high quality genomic DNA from animal tissues was introduced in this paper. It was suggested that this method could be extensively applied in the experiments of molecular biology.关键词:genomic DNA animal tissue PCR RAPD ...
Genomic DNA was extracted from lysed nuclei by organic phase separation (liquid/liquid extraction). This method has been successfully applied to the isolation and purification of DNA from eight different adult insects ( Heliothis zea, Musca autumnalis, M. domestica, Blatta orientalis, Tenebrio molitor...
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready fo
Isolation and characterization of the genomic dna 专利名称:Isolation and characterization of the genomic dna clones of ribosomal protein gene l25 in tobacco 发明人:LIFANG SHI,JOHNWAY GAO,BRIAN S.HOOKER,ZIYU DAI 申请号:AU1776901 申请日:20001120 公开号:AU1776901A 公开日:20010530 专利内容由知识...
Isolation of genomic DNA using magnetic cobalt ferrite and silica particles Adsorption separation techniques as an alternative to laborious traditional methods (e.g., based on phenol extraction procedure) have been applied for DNA purification. In this work we used two types of particles: silica and...
Genomic DNA of CTCs was subjected to whole genome amplification (WGA) followed by gene copy number analysis via array comparative genomic hybridization (aCGH). Results CTCs from nine (9) patients successfully profiled were observed to have multiple copy number aberrations including those previously ...