Protocol : Isolation of Genomic DNA from Laser- Microdissected TissuesDna, QiaampKit, MicroAe, Equilibrate BufferAw, BuffersRna, CarrierAl, BufferAtl, Buffer
If high-molecular-weight DNA is isolated for construction of genomic lambda libraries or genomic Southern blots the DNA should have a minimal size of 100-200 lb. For cosmid libraries larger DNA is needed. The protocol described here yields DNA of good quality with a standardized procedure ...
This paper extracts the genomic DNA of the young leaves of pea(Pisum sativum L.)by using an improved CTAB method,and makes the ultraviolet spectrophotometer and agarose gel electrophoresis.The results show that the obtained DNA,which presents better primary structure,higher purity and concentration,co...
Buccal DNA extraction kits Resources for genomic DNA isolation DNA & RNA Learning Center Nucleic Acid Purification and Analysis Support Center Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications. Protocol: Purification of genomic DNA using PureL...
A Protocol for Rapid and Inexpensive Genotyping of Mutant Mice from Dried Blood Spots: An Update have evaluated the efficiency of our method for the extraction of genomic DNA from DBS by testing its performance in genotyping mouse models of obesity and herein discuss the sensitivity, specificity an...
Trochetia species are known to synthesize a wide spectrum of polyphenols including flavonoids. These compounds represent a significant barrier to the extraction of pure genomic DNA. This study describes, for the first time, a reliable protocol for extracting good quality DNA in reasonable amount from...
J. & 30 DNA extraction from bas... GN Aristóteles,LL Clarice,RT Guerrero - 《Biotemas》 被引量: 73发表: 2005年 An inexpensive method for extraction of genomic DNA from fungal mycelia A rapid and efficient protocol for the extraction of genomic DNA from plant pathogenic fungi was developed...
Methods We developed a novel approach to isolate CTCs away from hematopoietic cells with high purity, enabling genomic analysis of these cells. The isolation protocol involves immunomagnetic enrichment followed by fluorescence activated cell sorting (IE/FACS). To evaluate the feasibility of isolation of...
摘要: A simple and rapid way for isolation of high quality genomic DNA from animal tissues was introduced in this paper. It was suggested that this method could be extensively applied in the experiments of molecular biology.关键词:genomic DNA animal tissue PCR RAPD ...
DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet ...