Genomic DNA Isolation from Tissue Samples and Library Prep for Tuba-Seq Barcode AnalysisThis protocol should be used in preparation from tumor barcode sequencing (Tuba-seq) with a goal of directly quantifying the tumor sizes within tissues. This protocol could also be used to quantify cells from ...
Current DNA isolation methods are limited in their ability to obtain quality and/or quantity DNA from plants, such as Emblica officinalis, Terminalia belerica, and Terminalia chebula, which have low pH and high amounts of secondary metabolites in tissue extracts. Our modified DNA isolation method ...
DNA was isolated from cultured mammalian cells, liver tissue and Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young ...
刘必谦等[36〕采用美国Roche公司的DNA Isolation Kit for Cell and Tissue和上海生工公司的UNIQ- 10小量柱式基因组DNA提取试剂盒对个体体积较小的圆紫菜(Porphyra suborbiculato )、铁钉菜(Ishige okamurae)等进行DNA 的提取,获得高纯度的基因组DNA,完全能满足酶切片段长度多态性(Amplified Fragment Length Polymorphi...
NucleoSpin Tissue (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K Notice to purchaser DocumentsYou May Also LikeImage Data 740952.250NucleoSpin® Tissue250 Preps* ...
E.Williams* and Pamela C.Ronald Department of Plant Pathology, University of California, Davis, CA 95616, USA Received April 5, 1994; Accepted April 15, 1994 A protocol is presented that utilizes potassium ethyl xanthogenate to liberate DNA from leaves without the need for tissue homogenization...
8 mM NaOH, 300–600 μl per 50–70 mg tissue or 107 cells 0.1 M or 1 M HEPES (free acid), see Table 1 Protocol notes Unless stated otherwise, conduct the procedure at room temperature. The molecular weight of the recovered DNA dep...
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants. Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. ... AA Alatar,MA Mahmoud,SA Al-Sohaibani,... -...
The average yield of extracted DNA varied from 50 to 100 ng of DNA per 100 mg of processed tissue, allowing over 150 PCR experiments. To investigate the effectiveness of our protocol, DNA extracts were subjected to different treatments, including restriction enzyme digestion, RAPD and ITS-PCR ...
Flow cytometry was used to analyse the DNA content of nuclei isolated from intact plant tissues and from callus and cell suspensioncultures invitro. Cell n... J. Dpooležel,Pavla Binarová,S. Lcretti - 《Biologia Plantarum》 被引量: 992发表: 1989年 Isolation of plant nuclei for estimation...