Cell-free nucleic acidsCerebrospinal fluidCirculating tumor DNADroplet digital PCRVacuumA standardized protocol for the isolation of cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is lacking. Therefore, we established a cfDNA isolation protocol optimized for clinical CSF specimens...
Cell isolation and cell separation using magnetic beads with Dynabeads magnetic separation technology provide a gentle, tube-based protocol to isolate high yields of pure, viable, and functional cells, ready to use for any downstream functional assays.
We recommend processing these types of samples using the manual protocol. Levels of cell-free DNA in circulation can range from 1 ng/mL to 100 ng/mL of plasma or serum depending on the donor. For samples containing low levels of cell-free DNA, we recommend increasing the starting sample ...
Cell isolation and cell separation using magnetic beads with Dynabeads magnetic separation technology provide a gentle, tube-based protocol to isolate high yields of pure, viable, and functional cells, ready to use for any downstream functional assays.
Table 2. Overview of cfDNA Isolation Kits KitMethodLysis step before cfDNA captureProtocol QIA Silica-based column Yes Manual PME Polymer-mediated enrichment No Manual RSC Magnetic beads Yes Automatic EQ Magnetic beads No Manual NpMV1/NpMV2 Magnetic beads Yes Manual cfDNA, cell-free DNA; EQ, Ep...
A comparison of cell-free DNA isolation kits:isolation and quantification of cell-free DNA in plasma. Sorber L,Zwaenepoel K,Deschoolmeester V,et al. The Journal of Molecular Diagnostics . 2017Sorber L, et al. A comparison of cell-free DNA isolation kits: isolation and quantification of ...
The Boykyo Cell-free DNA Blood Collection tubes can be used at room temperature between 6-37 C . Protocol Double spin protocols are commonly used to separate blood plasma for Cell‑Free DNA isolation. 300 x g for 20 min followed by 5000 x g for 10 min or 1600 x g for 10 min foll...
These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method.Introduction Circulating cell-free DNA (cfDNA) molecules are shed into bloodstream, plasma, serum, urine as well as other body fluids of humans [1]. Most evidence suggests that the released cfDNA ...
Cell-free DNA isolation from plasma Peripheral blood was collected from cancer patients using EDTA anticoagulant tubes. Plasma samples were isolated from whole blood using the UHN Biobank centrifugation protocol and stored at the UHN Biobank. 500 µl–1 ml plasma aliquots retrieved from the Bi...
Protocol Springer Protocols(2025) In Situ Isotope Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging Rosalie G. Rietjens,Bram Heijs Mass spectrometry imaging (MSI) allows for label-free spatial molecular interrogation of tissues. With advances in the field over recent years, the spatial...