Single-cell analysis is increasingly applied in biological and clinical studies with the improvement of single-cell methods. Here, we introduce a protocol for single cell isolation by flow cytometry. The main processes include tissue collection, preparation of single-cell suspensions, staining cells ...
The general framework of a scRNA-seq experiment consists of: single-cell isolation, cell lysis, mRNA capturing, mRNA reverse transcription into cDNA, cDNA amplification, library preparation, and sequencing [19]. Herein, we will review recent research in brain cells with scRNA-seq. In the first ...
Robotic Isolation Technology to discover single cell genetics PRECISION High resolution imaging combined with computer vision for cell selection EFFICIENCY Advanced micropipette technology for single cell manipulations RELIABILITY Onsite installation, training, technical support and free software upgrades ...
Moreover, the single-cell suspensions generated by using this protocol are compatible with droplet-based scRNA-seq technology, such as the 10x Genomics Chromium system. This protocol reliably produces single-cell suspensions from the placental tissues with high yield and viability for scRNA-seq. This...
Protocol Springer Protocols(2025) In Situ Isotope Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging Rosalie G. Rietjens,Bram Heijs Mass spectrometry imaging (MSI) allows for label-free spatial molecular interrogation of tissues. With advances in the field over recent years, the spatial...
For RNA and subsequent cDNA amplification, REPLI-g WTA Single Cell Kit (Qiagen, Cat.No.: 150063) was used with the Amplification of Total RNA from Single Cells' protocol according to the manufacturer’s guidelines with the exception that 3 μl Lysis buffer was added to 8 μl 1× Sin...
Comparison of Pala Single Cell Dispenser with traditional methods of sorting cells for clonal outgrowth. Pala Single Cell Dispenser, was used alongside limiting dilution (LDC) and FACS sorting to sort CHO host cells for a single cell cloning protocol sorting. Results highlight the higher cloning ef...
In this chapter, using lupus-prone MRL/ lpr mice, we describe a Breg cell isolation protocol for the purpose of single-cell RNA sequencing analysis. 展开 DOI: 10.1007/978-1-0716-3754-8_12 年份: 2024 收藏 引用 批量引用 报错 分享
Here, an optimized protocol for single nuclei isolation is presented for frozen and RNAlater preserved human kidney biopsies. The described protocol is fast, low cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Samples can be processed in 90 min or less....
TS and TG cells were directly lysed on plates at the indicated timepoints and total RNA prepared with an RNeasy MiniKit (Qiagen) with DNase treatment according manufacturers protocol. Microarray experiments were performed using Illumina Mouse WG-6 v2 Expression BeadChips. Microarray data was analysed...