Thus, it is essential to establish the parameters influenced by the isolation protocol in order to fully interpret T cell responses to antigens, chemokines, and cytokines.doi:10.1016/S0022-1759(02)00412-XFrédéric BernardSara JalecoValérie Dardalhon...
Figure 1.Typical EasySep™ Mouse T Cell Isolation Profile Starting with mouse splenocytes, the T cell content (CD3+CD19-) of the isolated fraction is 96.6 ± 2.0% (mean ± SD), using the purple EasySep™ magnet. Figure 2.Cell Isolation Protocol Lengths Typical time taken (in minutes...
IPHASE Human CD3+ T Cell Isolation Kit 规格:可分离PBMC数量 3*10^7 cells 试剂盒采用免疫磁珠阳性分选的方法,利用偶联于磁性微粒上人CD3单克隆抗体的高度特异性,使磁珠特异性结合PBMC(人外周血单个核细胞或脐带血单个核细胞)中的CD3+T细胞,通过外加磁场的作用,使得 CD3+T细胞得以滞留在磁场中而被分离出来。产品...
IPHASE Human CD11+ T Cell Isolation Kit 阳性分选 CD11+T细胞分选试剂盒 【试验原理】 免疫磁性细胞分选是用磁珠抗体偶联系统从样本细胞悬液中选择性吸附靶细胞的方法。其借助偶联于磁性微粒上抗体的高度特异性,识别并标记靶细胞的表面抗原,再通过外加磁场的作用,使结合了磁珠的靶细胞被吸附于磁场中,而未结合的...
Human Monocyte Cell Isolation Kits Human B Cell Isolation Kits Which Human CD8+ T Cell Isolation Product is Right for You? Highest purity for molecular assays Affordable & pure, flow compatible cells Extreme purity & physiological quality Untouc...
CD8+T细胞分离试剂盒用于从恒河猴PBMC中分离未经标记的CD8+T细胞。 非CD8+T细胞通过生物素偶联抗体和抗生物素微珠间接磁标记。 通过去除磁性标记的细胞可以获得高度纯净的未经标记CD8+T细胞。 下游应用 当直接标记T细胞表面分子可能干扰下游应用时,例如在细胞毒性T细胞活化研究中,将使用通过CD8+T细胞分离试剂盒分离的T细...
CD8+T细胞分离试剂盒用于从恒河猴PBMC中分离未经标记的CD8+T细胞。 非CD8+T细胞通过生物素偶联抗体和抗生物素微珠间接磁标记。 通过去除磁性标记的细胞可以获得高度纯净的未经标记CD8+T细胞。 下游应用 当直接标记T细胞表面分子可能干扰下游应用时,例如在细胞毒性T细胞活化研究中,将使用通过CD8+T细胞分离试剂盒分离的T细...
Sorted cells were collected using a FACSAria II cell sorter (Becton Dickinson). Isolation of total CD4+ T cells Total CD4+ T cells were isolated from cryopreserved PBMCs using magnetic negative selection as per the manufacturer’s protocol (Stem Cell Technologies, Vancouver, Canada). ...
8-Minute cell isolation kit using immunomagnetic negative selection Request Pricing Product Advantages Fast, easy-to-use and column-free Up to 98% purity with high recovery Untouched, viable cells What's IncludedProducts for Your Protocol To see all required products for your protocol, please ...
This protocol outlines a medium-throughput strategy based on combinatorial dye staining of pools of effector T cells to screen in parallel the reactivity of up to hundreds of T cell receptors against patient primary tissues or panels of antigens. ...