Mouse Spleen Cell Isolation Protocol Introduction The spleen is the site for hematopoiesis, red blood cell clearance, and immunologic functions, and therefore, a good source of cells. It filters cell debris, pathogens, and irregular cells. It is ...
Cite this protocol Stagg, A.J., Burke, F., Hill, S., Knight, S.C. (2001). Isolation of Mouse Spleen Dendritic Cells. In: Robinson, S.P., Stagg, A.J., Knight, S.C. (eds) Dendritic Cell Protocols. Methods in Molecular Medicine™, vol 64. Humana Press. https://doi.org/10.1...
Spleen cells from infected with virus were separated into subpopulations on the basis of buoyant density in discontinuous gradients of isotonic albumin or differential adherence of cells to plastic. At three different intervals after , a population of "less dense" cells was found in albumin gradients...
Figure 1.Typical EasySep™ Mouse B Cell Isolation Profile Starting with mouse splenocytes, the B cell content (CD19+CD3-) of the isolated fraction is 97.6 ± 1.7% (mean ± SD), using the purple EasySep™ Magnet. Figure 2.EasySep™ Cell Isolation Protocol Lengths ...
Spleen-derived murine γδ T cells can be effectively expandedin vitrowith the proposed expansion protocol.These cells show high IFNγ and Granzyme B production along with the novel finding IL-24 production after IL-2/αCD3 exposure.Expanded γδ T cells are highly cytotoxic against tumor cell ...
Mouse Bone Marrow Cell Isolation Introduction This protocol describes the isolation of cells from the marrow of bone [1,2]. As compared with whole blood, bone marrow yields a more complex set of cells, including hematopoietic stem cells–respo...
RNA isolation Total RNA (including miRNAs) was isolated from mouse spleen tissue using the miRNeasy mini kit (Qiagen, Valencia, CA, USA) following the manufacturer's protocol. Briefly, for each sample, around 30 mg of spleen tissue was placed in a 2-ml collection tube containing 700 μl ...
Isolation of Mouse T Cells fromSpleen or Lymph Node Leucocytes This protocol is based on 1 x 107leucocytes. It is scalable from 1 x 107-1 x 109cells, (see table 1). Transfer 100 μl (1 x 107) leucocytes in Buffer 1...
Dynabeads Mouse T-Activator CD3/CD28(10 ml)Mouse spleen or lymph node cells, mouse T cell clones, mouse CD4+ and CD8+ T cell subsets.Artificial antigen-presenting cells, optimized for mouse T cell activation and expansion. Also includes a new protocol for mouse Treg ex...
Please read the protocol in its entirety before starting. Supplies Required Reagents Cells derived from mouse or rat bone marrow, spleen, peripheral blood, or fetal liver. Mice are routinely used between 6-12 weeks. Iscove’s Modified Dulbecco’s Media (IMDM) ...