Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarizing cultures), or in medium supplemented with ImmunoCul...
货号:ABC-1129 价格:¥询价 规格: 1 vial 数量:-+ 产品描述 参考文献 资料下载 相关产品 产品描述/Products Description The Mouse CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD8+ T cells...
Here, we provide an improved culture protocol for immature nave mouse CD4 T cells, including details for splenocyte isolation, nave CD4 T-cell purification and differentiation, and functional evaluation via flow cytometry. This protocol can also be applied for immature human CD4 T cells. For ...
After incubation with hexahistidine-bovine serum albumin (HH-BSA) for 24 h, total RNAs were extracted from the splenocytes with RNAzol B (TEL-TEST, USA) according to the supplier’s protocol. RT-PCR was carried out using the RNA-PCR kit supplied from Takara Shuzo (Japan). IgM genes ...
Splenocyte Isolation and Osteoclast Differentiation Osteoclast progenitors were isolated from wild-type and mutant mouse spleens, homogenized by mechanical disrup- tion and passed through 70-mm filters. The filtrates were put into red cell lysis buffer (0.74% NH4Cl, 0.01mM Tris-HCl, 4 1C) and...
Understanding the cause vs consequence relationship of gut inflammation and microbial dysbiosis in inflammatory bowel diseases (IBD) requires a reproducible mouse model of human-microbiota-driven experimental colitis. Our study demonstrated that human fe
Importantly, 2-LTR circles were also detected in HIV-1-infected primary mouse T-cells derived from splenocyte pools of 3 BALB/c mice. Levels of 2-LTR circles were comparable (Fig. 3D; mouse donor pool #1) or ~16-fold higher (Fig. 3D; mouse donor pool #2) than in primary human ...
Splenocytes isolated from naive wild-type Helicobacter-negative (H À ) C57BL/6 mice were enriched for CD4 þ T cells by magnetic-activated cell sorted depletion of CD8a þ and B220 þ cells using biotinylated antibodies and streptavidin microbeads (Miltenyi Biotec, Bergisch Gladbach, ...
The lysis of RBC in splenocyte samples were performed by “ACK (Ammonium-Chloride-Potassium) lysis buffer”. Cell suspensions of mice were washed, centrifuged and solution filtered with 0.45 μm filter. Finally, lymphocytes were then resuspend at 5 × 106 cells/ml in complete RPMI...
Skin innervation is crucial for normal wound healing. However, the relationship between nerve receptors and wound healing and the intrinsic mechanism remains to be further identified. In this study, we investigated the role of a calcitonin gene-related p