Brown and white adipocytes were harvested from the interscapular and flank areas of C57BL6 mice, respectively. Adipocytes were cultured with syngeneic splenocytes after isolation and differentiation. Intermittent hypobaric conditions were generated using cyclic negative pressure application for 48 h in ...
An activator of protein kinase C, 12-0- tetradecanoylphorbol 13-acetate, had little effect in isolation but the combined application of these two agents greatly enhanced DNA synthesis. The natural mediators of these events are presumed to be inositol trisphosphate and diacylglycerol derived from ...
Isolation of splenic lymphocytes operating on ice: the spleens removed from the mice were cut into 2 mm × 2 mm squares and placed on a cell sorter sieve made from steel wire mesh and together placed over a 60 × 15 petri dish. Using a circular grinding motion, the pieces were pressed...