英文名称:Rat/Mouse Spleen Lymphocyte Isolation 英文同义词:Rat/Mouse Spleen Lymphocyte Isolation CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览Rat/Mouse Spleen Lymphocyte Isolation国外供应商中国供应商 Rat/Mouse Spleen Lymphocyte Isolation推荐供应商 ...
1. 1. The isolation and the subcellular fractionation of syngeneic mouse spleen and thymus lymphocytes are described. 2. 2. All the fractions were characterized by reliable marker enzymes. 3. 3. Two plasma membrane fractions (PM1 and PM2) were isolated from each cell type. The purest plasma ...
Eom (Cornell University) for providing the facility for performing lymphocyte isolation and cryosection. We thank K. A. Strednak and the Cornell Center for Animal Resources and Education for their animal care service. This research was supported from Basic Science Research Program through the ...
淋巴细胞(lymphocyte)是由淋巴器官(包括淋巴结、脾脏以及胸腺)产生的体积最小的白细胞。主要存在于淋巴管中循环的淋巴液中,是机体免疫应答功能的重要细胞成分,是淋巴系统几乎全部免疫功能的主要执行者,是对抗外界感染和监控体内细胞变异的一线“士兵”。淋巴细胞...
Objective:To observe the influence of T-lymphocyte subsets from mouse spleen cells treated with echinococcus granulosus fluid in vitro.Methods:After 72 h co-culture of spleen cells from BABL/c mouse and Hydatid fluid with different concentrations:100,200,300,500 μl,percentage of CD3+CD4+/CD3+...
tumor infiltrating lymphocyteFicoll- HypaquePercollconditioned mediumBy using enzyme digestion and Ficoll- Hypaque or Percoll discontinuous density methods, we have successfully obtained tumor-infiltrating lymphocytes (TIL) from mouse hepatoma. When analyzing the purity of TIL after separation, it was found...
Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator J. exp. Med. (1972) S. Gillis et al. T-cell lymphoma model for the analysis of interleukin-1 mediated T-cell activation M.F. Julius et al. A rapid method for the isolation of functi...
Spleen Lymphocyte Kinetics in Mice under Normal and Inflammatory Conditions : An Application of the Transgenic Mouse Expressing β-Galactosidase (ROSA 26)(... The purpose of this study was to develop a method for the measurement of the cell kinetics of spleen lymphocytes using the ROSA 26 transge...
RNA isolation Total RNA (including miRNAs) was isolated from mouse spleen tissue using the miRNeasy mini kit (Qiagen, Valencia, CA, USA) following the manufacturer's protocol. Briefly, for each sample, around 30 mg of spleen tissue was placed in a 2-ml collection tube containing 700 μl ...
To measure T cell differentiation in vitro, naïve CD4+ T-cells were isolated from spleen, using a naïve CD4+ T-cell isolation kit according to the manufacturer's instructions (Miltenyi Biotec). Cells were stimulated with plate-bound anti-CD3 (5 μg/ml) and anti-CD28 (5 μg/ml...