Single cell isolationFlow cytometryFluorescence-activated cell sortingStaining cellsSingle-cell analysis is increasingly applied in biological and clinical studies with the improvement of single-cell methods. Here, we introduce a protocol for single cell isolation by flow cytometry. The main processes ...
Reduced expression of nucleolar genes induces stress and DNA damage. Here, we present a protocol to analyze DNA fragmentation at the single-cell level in Drosophila imaginal discs using an optimized Alkaline Comet Assay. We describe steps for larvae development, tissue disaggregation, and single cell...
Background: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This leads to inconsistency of the results and limits the comparison of the data from different laboratories. Our aim was to ...
Receptor 2 of the human epidermal growth factor (HER2/neu, c-erbB2) is a 185 kDa proto-oncogene protein characterized by an overexpression in some oncological diseases, including 30% of mammary glands cancers, as well as tumors in the ovary, stomach and other organs of the human body. Sinc...
Here, an optimized protocol for single nuclei isolation is presented for frozen and RNAlater preserved human kidney biopsies. The described protocol is fast, low cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Samples can be processed in 90 min or less....
Here, we describe a protocol for the EPAM-ia method, which is based on flow cytometry for simultaneous isolation and analysis of endothelial cells, pericytes, astrocytes and microglia. This method is based on differential processing of NVU cell types using enzymes, mechanical homogenization and ...
We show that concentration of cell culture conditioned media using ultrafiltration devices results in increased vesicle isolation when compared to traditional ultracentrifugation protocols. However, our data on using conditioned media isolated from the Non-Small-Cell Lung Cancer (NSCLC) SK-MES-1 cell ...
Bone marrow cell isolation Timing: 3 h The following steps provide the procedure of collection and differentiation of bone marrow-derived cells into BMDCs. Note: Ensure that all the reagents and samples are kept on ice during the entire procedure. Perform all steps in a laminar flow hood with...
proteomics and cell culture. Multiple cell-type analyses using UpSet can then be applied to obtain robust targets from single or multiple NVU cell types in neurological diseases associated with BBB dysfunction. The EPAM-ia method is also amenable to isolation of several other cell types, including...
1Department of Anatomy, Korea University College of MedicineProtocol 1.材料和培养基的制备 为SCZ的解剖和离解,包住大脑基质,双刃刀片,并用铝箔钳子,然后通过高压消毒他们。 制备50毫升冷PBS缓冲液洗涤全鼠脑。 设置一个解剖显微镜和制备用于脑(蒸压剪刀和镊子)的解剖所需的外科手术工具和SCZ(1ml注射器,地下...