DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet ...
DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet is ...
Protein-Protein Interactions (PPIs) are fundamental means of functions and signalings in biological systems. The massive growth in demand and cost associated with experimental PPI studies calls for computational tools for automated prediction and underst
Ninety-six-well plates were coated with 0.05 mg ml−1poly-d-lysine (BD 354210) one day prior to isolation. A dissection solution was made using HBSS (ThermoFisher 14025092) supplemented with 10 mM HEPES (ThermoFisher 15630080), 33 mMd-glucose (Sigma-Aldrich G8270) and 43 mM...
This protocol will allow the isolation of antibody-based degraders that works in the intracellular environment. Hence, it will be possible to use the antibody without the E3 ligase as tracking or inhibitory tools rather than only being a degrader. In addition, the antibody-based degraders can be...
the proteins form a pellet at the bottom of the tube. The supernatant can then be both dried down and reconstituted or can be directly injected onto theLCsystem forMSanalysis.Zinc sulfateis also commonly added to the organic solvent. This procedure was initially described when using whole blood...
The mRNA expression of activating tran- scription factor- (ATF-) 6 and Krüppel-like factor- (KLF-) 6 in HLE-B3 cells was analyzed using an ABI 7500 real- time PCR system (Applied Biosystems, Foster City, CA, USA). The cells were collected and examined by RT-PCR. The sequences of...
The protocol for total protein extraction from mammalian cells consists of culturing and harvesting the cells, pipetting the sample into clean micro centrifuge tubes, and immediately placing on ice. The tube is then centrifuged to pelle...
be predicted at an error rate of 1%, this results in the structure of 19,842 human heterodimeric protein structures. The computational cost to run all of this is ~5 days on an Nvidia A100 system and has since the development of the pipeline presented here, deemed FoldDock, been applied37...
RNA from cultured cells was extracted using the mirVana™ miRNA Isolation Kit (Ambion, Austin, TX, USA). mRNA expression values were measured in triplicate using the Roche LightCycler 480 and normalized to b-actin expression as a housekeeping control. Expression values were calculated according to...