In principle, quantification of intact protein species with mass spectrometry should be the most appropriate method, since with high-resolution mass spectrometers available today relatively small differences in the molecular weights of protein species are detectable. In combination with immuno-affinity method...
Figure 1.Structural templating and nucleated polymerization.(a)Principle of structural templating. Pre-existing structure (represented by squares) directs conversion of the intermediate (circles) into the same type of structure.(b)Nucleated polymerization model for structural templating, as applied to pri...
In doing so, PSMs with higher co-isolation and lower average signal to noise were selectively removed where these low-quality PSMs disagreed with other PSMs. Remaining PSMs were then median center normalized. Protein-level abundances were estimated by summing PSM-level abundances, for proteins with...
Having established the orthogonality of the dissociable ribosome in the OSYRIS cells, we carried out a proof-of-principle experiment to test the potential of the system for selecting mutations in the rRNA of the large subunit that would enable the ribosome to carry out otherwise problematic tasks....
& Perr, M. Isolation of proteins and protein complexes by immunoprecipitation. Methods Mol. Biol. 424, 349–364 (2008). Aronheim, A., Zandi, E., Hennemann, H., Elledge, S. J. & Karin, M. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. ...
The last three chapters are on proteins which are the workhorses in the cell. The most fundamental property of a protein is its isoelectric point (pI) which determines what cellular components a protein can interact electrostatically (e.g., a positively
Isolation-based anomaly detection. ACM Trans Knowl Discov Data. 2012;6(1):3. 96. Xue LC, Dobbs D, Honavar V. HomPPI: a class of sequence homology based protein–protein interface prediction methods. BMC Bioinformatics. 2011;12(1):244. 97. Jordan RA, Yasser EM, Dobbs D, Honavar V. ...
consequence, the many proteins thus associated with a liposome collectively have a broad variety of affinity interactions with the lipid shell, resulting in many distinct residence times of the different proteins at the liposome surface.22Thus, a huge number of equilibrium constants (in principle, ...
enhancedinternalizationof G protein-coupled receptors such as the β-adrenergic receptor following receptor stimulation. This results in a down-regulation of receptor number in the plasma membrane and consequently in a prolonged desensitization of the system (Drake et al., 2006; Heck and Bylund, ...
The present invention relates to a method for the isolation of proteins that comprise disulfide bonds in their native conformation. Essentially, a method of the present invention ma