Protein-Protein Interactions (PPIs) are fundamental means of functions and signalings in biological systems. The massive growth in demand and cost associated with experimental PPI studies calls for computational tools for automated prediction and underst
the proteins form a pellet at the bottom of the tube. The supernatant can then be both dried down and reconstituted or can be directly injected onto theLCsystem forMSanalysis.Zinc sulfateis also commonly added to the organic solvent. This procedure was initially described when using whole blood...
The principle behind MD is to represent computationally molecules and ions, which can be at an atomistic level or coarse-grained; define a volume for the experiment to take place, which will be represented as a box, and apply Newtonian physics principles in the system to calculate all bonded ...
The diffusion-induced phase separation process involves the conversion of a film of polymer solution cast on a suitable substrate into a two-phase system. By immersing the substrate in a non-solvent bath, the solvent in the casting solution is exchanged with non-solvent, and the precipitation ...
In principle, quantification of intact protein species with mass spectrometry should be the most appropriate method, since with high-resolution mass spectrometers available today relatively small differences in the molecular weights of protein species are detectable. In combination with immuno-affinity method...
Yet, it is still difficult to estimate whether membrane proteins are patterned into general protein-rich domains in living cells, or whether this is only a membrane isolation artefact. Although this type of membrane arrangement has been supported by several live-cell single-molecule imaging studies...
MS charting can in principle be applied in many of the same ways that two-dimensional electrophoresis is currently used, but because molecular speciÐcity is integral to MS charting, the latter adds a beneÐt to the analysis that is unattainable with electrophoresis alone. Applications for MS...
HCD MS/MS spectra were acquired in the linear ion trap using a quadrupole isolation window of 1.6 Da, an AGC target value of 5E3, and a normalized collision energy of 30%. In data-dependent acquisition (DDA) MS/MS measurements, many of the (often low-intensity) proteotypic frameshift-...
the cytoplasm using the CyDisCo system.BModification process of phosphorylation and acetylation. P: phosphonate; AC: acetyl.CModification process of glycosylation by overexpression of a heterologous N/O-glycosylase.DIntroduction of PTMs via ncAA. The figure shows the principle of phosphoserine ...
Total RNA was isolated with Promega's SV Total RNA Isolation System. RT-PCR was performed using a TITANIUM One-Step RT-PCR Kit (BD Biosciences, Mountain View, CA), and 500 ng of template. The following pair of primers were used: G3 (5'-GGTGATCACGTTTCACAATGAAGCCAGG-3')/G4 (5'-...