‹Protein-Protein Interactions Support Protein-Protein Interactions Support—Getting Started Protein-Protein Interactions Support—Troubleshooting Find valuable information. Optimize your protein-protein interaction experiments ...
Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support—Troubleshooting Find valuable information for your protein IP, Co-IP, and pulldown experiments. Optimize your protein immu...
As with other chromatographic methods, ion exchange chromatography may require some troubleshooting to determine the optimum conditions for protein binding, protein elution, and adequate resolution. In some cases, a pure protein may exhibit multiple chromatographic peaks due to the presence of more than...
The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant ...
Western blot troubleshooting Western blot using IgY Other protocols Dot blot ELISA Immunoprecipitation Immunoprecipitation/IgY Immunohistochemistry Anti-KLH antibody removal Yolk delipidation Meiotic staining Technical information Antibody types IgG IgM IgY ...
Blots were washed 6 × 10 minutes in 0.1 M PBS and subsequently imaged using an Odyssey Infrared Imaging System and quantified using Image Studio Software (Image StudioTM Lite, Lincoln, NE). Due to alteration in the expression levels of many standard loading control proteins in ...
troubleshooting guide are provided. This is a preview of subscription content,access via your institution Access options Subscription info for Chinese customers We have a dedicated website for our Chinese customers. Please go tonaturechina.comto subscribe to this journal....
The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant ...
Troubleshooting strategies or how much time should be spent before moving to the next option PANELISTS: Oleg Brodsky, MBA, Senior Principal Scientist, Structural Biology & Protein Sciences, Pfizer Inc. Christa Cortesio, PhD, Director, Protein Biochemistry & Analytics Core, Kite, A Gilead...
The study of membrane proteins, such as G protein-coupled receptors (GPCRs), can be challenging, due to the difficulty in preserving the correct conformation of the protein in isolation from its native environment, as well as establishing the right experimen...