On the individual protein level, isolation methods, such as co-immunoprecipitation17, pull-down18, and crosslinking19 are used to establish the structures of individual proteins, so that surficial ‘hotspots’ can be located and analyzed. In short, hierarchy knowledge of structure information is ...
The total volume of each sample was injected onto an Acquity UPLC BEH C18 column (2.1-mm inner diameter × 150 mm; 1.7-μm particle size) on an Acquity UPLC System with a diode array detector (Waters), and the peptides were eluted from the column using a linear gradient of 4...
Isolation and Analysis of Lipopeptides and High Molecular Weight Biosurfactants. In Handbook of Hydrocarbon and Lipid Microbiology; Springer: Berlin/Heidelberg, Germany, 2010; pp. 3688–3704. [Google Scholar] Navon-Venezia, S.; Zosim, Z.; Gottlieb, A.; Legmann, R.; Carmeli, S.; Ron, E....
(BADH) fusion protein increased by over 120% while the activity of the free enzyme system increased only 45%. Importantly, the activity of theBADH enzymeremained constant when studied in isolation, despite the increase in the viscosity of solution [45]. These studies suggest that the benefit ...
More recently approaches to identifying whether the fetus has a D genotype have involved the isolation of cell free fetal DNA (cffDNA) from maternal plasma in early pregnancy and this has led to new avenues for diagnosis, monitoring, and administration of prophylactic anti-D in pregnant women. ...
Application of RPISeq to constructing RNA-protein interaction networks Encouraged by the success of RPISeq in predicting specific RPIs, we examined its effectiveness in constructing RNA-protein interaction networks in several model organisms, using only information derived from RNA and protein sequences. ...
Isolation of non-ruptured protoplasts through the use of a sucrose gradient significantly aids the identification of protoplast populations with and without GFP-expression. Once an initial delineation of the protoplast populations on the cell sorter has been established, this step may potentially be ...
Performance comparison on TAIR’s 5-star System In order to assess the performance of the annotation pipelines on groups of genes constructed from varying levels of evidence, we used the TAIR10 5-star system. TAIR associates a quality score to eachA. thalianatranscript based on the evidence use...
Testing of fluidic isolation Isolation of the different compartments in microfluidic conditions without cells was also assessed using either Coomassie Blue or LVs particles. Coomassie Blue (2%) or LVs particles (400 ng p24) diluted in PBS were added to the somatodendritic compartment of microfluidic ...
(Figs.2a,dand3b–dand Extended Data Figs.5dand6e,f). Finally, we demonstrated the application of PVCs as delivery tools in diverse contexts, such as in the specific killing of cancer cells or as mediators of genome editing (Fig.2a–d), and we showed that the system operates as ...